{"id":"e1cd012a-40c1-5150-beb6-8adfc855688e","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zip6-influx","predicate":"supports","statement":"T-cell receptor activation raised cytoplasmic zinc within one minute, especially near the receptor-contact region; the rise depended on extracellular zinc and was attenuated by ZIP6 suppression.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"288219de-7aad-52e0-8aa4-8bcdc9e7304c","mechanism_event_label":"ZIP6 helped generate a rapid local zinc signal after receptor activation.","subject":{"id":"517e6e15-f4f2-579f-b4e8-b5a5a40345f1","slug":"slc39a6","display_name":"Human zinc transporter ZIP6 / SLC39A6","entity_type_key":"protein"},"object":{"id":"85a9e08c-68ab-56bb-a575-313e97c80a37","slug":"cytosolic-labile-zinc","display_name":"Cytosolic labile zinc availability","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"288219de-7aad-52e0-8aa4-8bcdc9e7304c","stable_key":"6d38d43e-01e4-5641-93be-65654271e242:zn-sig-zip6-influx-event","event_type":"biochemical_relationship","label":"ZIP6 helped generate a rapid local zinc signal after receptor activation.","description":"T-cell receptor activation raised cytoplasmic zinc within one minute, especially near the receptor-contact region; the rise depended on extracellular zinc and was attenuated by ZIP6 suppression.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"transported_ion","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"517e6e15-f4f2-579f-b4e8-b5a5a40345f1","slug":"slc39a6","display_name":"Human zinc transporter ZIP6 / SLC39A6","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"85a9e08c-68ab-56bb-a575-313e97c80a37","slug":"cytosolic-labile-zinc","display_name":"Cytosolic labile zinc availability","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Zinc(II) ion (transported_ion)","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/zinc-signaling-sources/21422171-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"e3b0e519eb5c44185655c0195cb81c713f2b005d70a982466e3e041e997fae59\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human T-cell receptor signaling study","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Receptor activation, extracellular zinc availability and ZIP6 suppression comparisons.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Culture zinc availability and local signals cannot be mapped directly to oral doses or plasma thresholds. Calcium fluorescence tracks the signal rather than proving one specific calcium channel mechanism.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Zinc research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"zinc","display_name":"Zinc","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ZIP6 helped generate a rapid local zinc signal after receptor activation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. (2011). https://pubmed.ncbi.nlm.nih.gov/21422171/ DOI: 10.1084/jem.20100031","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"CD4 T cells at the receptor-contact region","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"e65f2ef6-634c-5dc0-a9fe-dc4ffe357411","evidence_kind":"source_excerpt","locator":"Lines 1013-1025","start_line":1013,"end_line":1025,"excerpt":"### zn-sig-zip6-influx\nT-cell receptor activation raised cytoplasmic zinc within one minute, especially near the receptor-contact region; the rise depended on extracellular zinc and was attenuated by ZIP6 suppression.\nCondition category: normal\nnutrient_topic: Zinc research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ZIP6 helped generate a rapid local zinc signal after receptor activation.\norganism: Homo sapiens\ntissue_or_cell_type: CD4 T cells at the receptor-contact region\nexperimental_model: Primary human T-cell receptor signaling study\nlimitations: Culture zinc availability and local signals cannot be mapped directly to oral doses or plasma thresholds. Calcium fluorescence tracks the signal rather than proving one specific calcium channel mechanism.\nexposure: Receptor activation, extracellular zinc availability and ZIP6 suppression comparisons.\ncross_nutrient: Zinc(II) ion (transported_ion)\nevidence_span: {\"source_cache\": \"artifacts/zinc-signaling-sources/21422171-abstract.txt\", \"locator\": \"Primary indexed abstract\", \"file_sha256\": \"e3b0e519eb5c44185655c0195cb81c713f2b005d70a982466e3e041e997fae59\"}\n[zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. (2011). https://pubmed.ncbi.nlm.nih.gov/21422171/ DOI: 10.1084/jem.20100031","model_system":"Primary human T-cell receptor signaling study","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [zn-sig-21422171] Regulation of T cell receptor signaling by activation-induced zinc influx. 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