{"id":"e1bb6211-7ffa-5683-a292-fe85c143e057","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-amt-onecarbon","predicate":"catalyzes-formation-of","statement":"Human AMT transfers the aminomethyl-lipoyl intermediate's carbon to tetrahydrofolate, yielding 5,10-methylene-THF, ammonia and reduced H-protein.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"86a7419f-7f01-54b2-8f57-c14c6568a226","mechanism_event_label":"Glycine cleavage connects its B6-dependent first step to a separate folate-dependent step.","subject":{"id":"80dafa0c-77d1-5bc3-b983-62bff2dabc75","slug":"amt","display_name":"Human aminomethyltransferase / glycine-cleavage T-protein","entity_type_key":"protein"},"object":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"86a7419f-7f01-54b2-8f57-c14c6568a226","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-amt-onecarbon-event","event_type":"biochemical_relationship","label":"Glycine cleavage connects its B6-dependent first step to a separate folate-dependent step.","description":"Human AMT transfers the aminomethyl-lipoyl intermediate's carbon to tetrahydrofolate, yielding 5,10-methylene-THF, ammonia and reduced H-protein.","status":"provisional","compartment":{"slug":"mitochondrial-matrix","display_name":"Mitochondrial matrix"},"participants":[{"entity":{"id":"80dafa0c-77d1-5bc3-b983-62bff2dabc75","slug":"amt","display_name":"Human aminomethyltransferase / glycine-cleavage T-protein","entity_type_key":"protein"},"role":"folate-dependent enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"78a93278-7e43-59c7-b097-31bbae21c771","slug":"gcsh","display_name":"Human glycine-cleavage H-protein / GCSH","entity_type_key":"protein"},"role":"lipoyl carrier protein","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"10a6d69a-18d4-5891-b3f7-406ecbdf0fb6","slug":"gcsh-aminomethyl-lipoyl","display_name":"Human GCSH aminomethyl-lipoyl intermediate","entity_type_key":"protein_state"},"role":"substrate state","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"d2b23343-c688-5fc3-9f42-bfac0050552b","slug":"tetrahydrofolate","display_name":"Tetrahydrofolate","entity_type_key":"small_molecule"},"role":"one-carbon acceptor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"0413b195-325e-5757-a72b-f445d65c17bc","slug":"5-10-methylenetetrahydrofolate","display_name":"5,10-Methylenetetrahydrofolate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"1374ec24-1a5b-552b-982f-51f9082f4187","slug":"ammonia","display_name":"Ammonia","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"fccfb9d6-ed19-5970-9237-59ff5d28a743","slug":"gcsh-dihydrolipoyl","display_name":"Human dihydrolipoyl GCSH","entity_type_key":"protein_state"},"role":"product state","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""},{"entity":{"id":"f4497497-e8be-5658-af01-2a75e9802027","slug":"gldc","display_name":"Human glycine decarboxylase / GLDC","entity_type_key":"protein"},"role":"upstream PLP enzyme","stoichiometry":null,"state_label":"","sequence_order":7,"notes":""},{"entity":{"id":"640b6164-093b-5297-a6bf-55ac8da852ae","slug":"dld","display_name":"DLD","entity_type_key":"protein"},"role":"downstream lipoyl reoxidation enzyme","stoichiometry":null,"state_label":"","sequence_order":8,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"B6-dependent GLDC precedes lipoyl-carrier transfer and folate-dependent AMT chemistry.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human AMT structures and mutational analyses","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"AMT is the directly examined human enzyme; this study did not test dietary B6 depletion or the entire pathway flux.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Glycine cleavage connects its B6-dependent first step to a separate folate-dependent step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-amt-2005] Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia. (2005). https://pubmed.ncbi.nlm.nih.gov/16051266/ DOI: 10.1016/j.jmb.2005.06.056","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"504ad6ae-2380-54fb-93dc-76b55c1aa89b","evidence_kind":"source_excerpt","locator":"Lines 682-692","start_line":682,"end_line":692,"excerpt":"### b6-met-amt-onecarbon\nHuman AMT transfers the aminomethyl-lipoyl intermediate's carbon to tetrahydrofolate, yielding 5,10-methylene-THF, ammonia and reduced H-protein.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Glycine cleavage connects its B6-dependent first step to a separate folate-dependent step.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Purified human AMT structures and mutational analyses\nlimitations: AMT is the directly examined human enzyme; this study did not test dietary B6 depletion or the entire pathway flux.\ncross_nutrient: B6-dependent GLDC precedes lipoyl-carrier transfer and folate-dependent AMT chemistry.\n[b6-amt-2005] Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia. (2005). https://pubmed.ncbi.nlm.nih.gov/16051266/ DOI: 10.1016/j.jmb.2005.06.056","model_system":"Purified human AMT structures and mutational analyses","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-amt-2005] Crystal structure of human T-protein of glycine cleavage system at 2.0 A resolution and its implication for understanding non-ketotic hyperglycinemia. (2005). https://pubmed.ncbi.nlm.nih.gov/16051266/ DOI: 10.1016/j.jmb.2005.06.056","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}