{"id":"e10a8445-3986-5b3a-889a-c7e75dc845f8","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-pip2-recovery","predicate":"prevents_recovery_of","statement":"Blocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"e5b7d2c2-0b53-59aa-9e40-f59724bb633b","mechanism_event_label":"Blocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.","subject":{"id":"229dc6db-121e-58c0-af0e-2971cb975613","slug":"phosphatidylinositol-4-kinase-blockade","display_name":"Experimental phosphatidylinositol-4-kinase blockade","entity_type_key":"cellular_process"},"object":{"id":"fa22b5b0-aba8-545a-9e25-5a8fff2713b9","slug":"desensitized-trpv1-recovery","display_name":"Functional recovery of desensitized TRPV1","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e5b7d2c2-0b53-59aa-9e40-f59724bb633b","stable_key":"49fecf36-4958-5f15-8c78-9a68cba50763:capsaicin-pip2-recovery-event","event_type":"observed_relationship","label":"Blocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.","description":"Blocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"229dc6db-121e-58c0-af0e-2971cb975613","slug":"phosphatidylinositol-4-kinase-blockade","display_name":"Experimental phosphatidylinositol-4-kinase blockade","entity_type_key":"cellular_process"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"fa22b5b0-aba8-545a-9e25-5a8fff2713b9","slug":"desensitized-trpv1-recovery","display_name":"Functional recovery of desensitized TRPV1","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9cdd3a64-5185-5998-82a6-d25c8409b998","slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"8e0c61d8-3930-51d5-bce2-25a5edfb797c","slug":"human-trpv1","display_name":"Human transient receptor potential vanilloid 1 / TRPV1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"5314e047-ba5d-52bb-8cfa-df41b6428824","slug":"pip2","display_name":"Phosphatidylinositol 4,5-bisphosphate / PIP2","entity_type_key":"lipid"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Prolonged capsaicin followed by lipid-kinase blockade","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Recovery interval","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; model-specific source-derived curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"TRPV1-expressing cells with Kir2.1 PIP2 biosensor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High intracellular ATP and PIP2 resynthesis were required; this is an experimental machinery perturbation, not evidence that dietary ATP supplementation changes analgesia.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Capsaicin chapter; interacting nutrients, drugs, peptides and proteins retain their experimental settings.","comparator":null,"unit":null,"notes":"","entity":{"slug":"capsaicin","display_name":"Capsaicin","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"TRPV1-expressing cells with Kir2.1 PIP2 biosensor","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Blocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional recovery from desensitization of vanilloid receptor TRPV1 requires resynthesis of phosphatidylinositol 4,5-bisphosphate. (2005). https://pubmed.ncbi.nlm.nih.gov/15888659/ DOI: 10.1523/JNEUROSCI.1296-05.2005","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Channel recovery after desensitization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"80b5a00a-8720-5568-af27-d9fb7f46c223","evidence_kind":"source_excerpt","locator":"Lines 44-53","start_line":44,"end_line":53,"excerpt":"## capsaicin-pip2-recovery\nBlocking phosphatidylinositol-4-kinase prevented recovery of TRPV1 after prolonged capsaicin desensitization, linking recovery to PIP2 resynthesis.\nModel/species: TRPV1-expressing cells with Kir2.1 PIP2 biosensor\nTissue/system: Channel recovery after desensitization\nExposure: Prolonged capsaicin followed by lipid-kinase blockade\nRoute: In vitro\nDuration: Recovery interval\nLimits: High intracellular ATP and PIP2 resynthesis were required; this is an experimental machinery perturbation, not evidence that dietary ATP supplementation changes analgesia.\nPrimary reference: Functional recovery from desensitization of vanilloid receptor TRPV1 requires resynthesis of phosphatidylinositol 4,5-bisphosphate. (2005). https://pubmed.ncbi.nlm.nih.gov/15888659/ DOI: 10.1523/JNEUROSCI.1296-05.2005\nAccess: Primary PubMed abstract and indexed metadata reviewed. Full-text method details not stated here remain unresolved.","model_system":"TRPV1-expressing cells with Kir2.1 PIP2 biosensor","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span with primary citation; not a publisher quotation.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"883c8afb-af48-5561-ae3b-00d4f3c26752","stable_key":"import-49fecf36-4958-5f15-8c78-9a68cba50763","title":"Capsaicin: mechanism of action and interactions (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted source-specific curation with primary-study citations, model, exposure, route, duration, negative findings and limitations preserved. Not publisher full text.","file_path":"","sha256":"ea5c8dc49ffdfcec3f5542fed8be406c4af37873b76416910f88a743b733466e","revision_id":"79e49bef-520e-5503-ad2e-7de89ad236cf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}