{"id":"e0f741b7-7c6c-58e5-aa53-2ee8ce03d77e","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-spermine-nmda","predicate":"potentiates_despite_agmatine","statement":"Spermine still potentiated NMDA current in the presence of agmatine, supporting distinct interaction sites for the two compounds.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"a3354574-46c2-5d55-af2f-7689e66aa2f2","mechanism_event_label":"Two connected polyamine-related molecules can push the same channel in different directions.","subject":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"object":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"a3354574-46c2-5d55-af2f-7689e66aa2f2","stable_key":"220adaab-10d8-5c4e-a3f5-a48700ea794d:agmatine-sulfate-spermine-nmda-event","event_type":"observed_relationship","label":"Two connected polyamine-related molecules can push the same channel in different directions.","description":"Spermine still potentiated NMDA current in the presence of agmatine, supporting distinct interaction sites for the two compounds.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ded80d0b-9076-5034-9e87-cd38f7dafae5","slug":"spermine","display_name":"Spermine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5050009a-583a-5618-bc59-ef31f4014224","slug":"rat-nmda-receptors","display_name":"Rat NMDA-type glutamate receptors, subunits unresolved","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae2dddf4-ce04-57e3-8a06-7ed9fdc24554","slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e2f83eb4-99ed-5050-87d3-3e4701d05c1a","slug":"agmatine","display_name":"Agmatine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rat hippocampal-neuron electrophysiology.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This is a cellular interaction, not evidence that co-supplementation is beneficial.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Agmatine Sulfate collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"agmatine-sulfate","display_name":"Agmatine Sulfate","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Two connected polyamine-related molecules can push the same channel in different directions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Agmatine selectively blocks the N-methyl-D-aspartate subclass of glutamate receptor channels in rat hippocampal neurons. · 1999 · https://pubmed.ncbi.nlm.nih.gov/9918557/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fe86b3c1-980a-51d6-bf50-154bf99bf900","evidence_kind":"source_excerpt","locator":"Lines 268-274","start_line":268,"end_line":274,"excerpt":"## agmatine-sulfate-spermine-nmda\nTwo connected polyamine-related molecules can push the same channel in different directions.\nSpermine still potentiated NMDA current in the presence of agmatine, supporting distinct interaction sites for the two compounds.\nModel: Rat hippocampal-neuron electrophysiology.\nLimitations: This is a cellular interaction, not evidence that co-supplementation is beneficial.\nEvidence access: Primary abstract\nAgmatine selectively blocks the N-methyl-D-aspartate subclass of glutamate receptor channels in rat hippocampal neurons. · 1999 · https://pubmed.ncbi.nlm.nih.gov/9918557/","model_system":"Rat hippocampal-neuron electrophysiology.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e0426971-7d13-51c9-8354-6547b664f1cf","stable_key":"import-220adaab-10d8-5c4e-a3f5-a48700ea794d","title":"Agmatine Sulfate: transport, guanidino metabolism, ion channels and cross-nutrient mechanisms (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b55d70e22d07b80964f825770d92fdd1745221420ed8af8d94ca5182b9994509","revision_id":"fc7be029-20e0-5943-b74e-3b06ba694571","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}