{"id":"e0ddd315-8276-5d22-9cda-3a830c63d7e9","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-sirt3-nad-retained","predicate":"increases_in_recorded_comparison","statement":"The NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"912ede2b-0e90-552c-b935-89322521d324","mechanism_event_label":"The NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.","subject":{"id":"6d70addf-e302-5605-9c6a-02358209c876","slug":"nmn-apigenin-combination-2026","display_name":"NMN plus apigenin preparation in the 2026 musculoskeletal study","entity_type_key":"chemical_species"},"object":{"id":"6a1be942-8261-5ba7-9d98-2a50c0d51fc3","slug":"circulating-nad-content","display_name":"Circulating NAD content","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"912ede2b-0e90-552c-b935-89322521d324","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-sirt3-nad-retained-event","event_type":"experimental_observation","label":"The NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.","description":"### api-nmn-sirt3-nad-retained\n\nThe NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.\nPrecursor supply and downstream response are different steps.\nModel and exposure: Aged Sirt3-null mice; main Figure 7.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | 2026 | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468 | Access: Primary main-text Results and figure descriptions reviewed. Detailed supporting Methods/supplements inaccessible; exact doses and preparation details not independently extracted.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6d70addf-e302-5605-9c6a-02358209c876","slug":"nmn-apigenin-combination-2026","display_name":"NMN plus apigenin preparation in the 2026 musculoskeletal study","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"Combined preparation in Sirt3-null mice","sequence_order":0,"notes":""},{"entity":{"id":"6a1be942-8261-5ba7-9d98-2a50c0d51fc3","slug":"circulating-nad-content","display_name":"Circulating NAD content","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"measured pool","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary main-text Results and figure descriptions reviewed. 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Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-nmn-sirt3-nad-retained; Precursor supply and downstream response are different steps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"48a1694b-eb91-58dc-9a1e-676af3ac4ff3","evidence_kind":"source_excerpt","locator":"Lines 556-562","start_line":556,"end_line":562,"excerpt":"### api-nmn-sirt3-nad-retained\n\nThe NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.\nPrecursor supply and downstream response are different steps.\nModel and exposure: Aged Sirt3-null mice; main Figure 7.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | 2026 | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468 | Access: Primary main-text Results and figure descriptions reviewed. Detailed supporting Methods/supplements inaccessible; exact doses and preparation details not independently extracted.","model_system":"Aged Sirt3-null mice; main Figure 7.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. 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No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[{"id":"d560466a-ceaa-5d27-9428-677cbb5b0de5","relation_type":"limits_scope_of","notes":"NAD supply can remain responsive while a downstream dependency weakens outcomes.","source_claim_id":"e0ddd315-8276-5d22-9cda-3a830c63d7e9","source_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-sirt3-nad-retained","source_statement":"The NMN/apigenin-associated blood NAD+ and NAD+/NADH increases persisted in Sirt3-knockout mice.","target_claim_id":"0d5885e5-0120-5494-98ab-14344813fb70","target_claim_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-sirt3-benefit-attenuated","target_statement":"Sirt3 deletion partially attenuated the structural and functional benefits of NMN plus apigenin."}],"conflicts":[],"corrections":[],"research":null}