{"id":"e0b51705-514e-528e-bfb8-0ff55c1a8dd5","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-metal-rescue-zinc-ion","predicate":"increases_in_recorded_experiment","statement":"Added zinc-ion reversed the relevant metal-defence-mutant hypersensitivity to allicin in yeast.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"8469972f-a08d-55a4-857c-c0f9e376ce1b","mechanism_event_label":"Added zinc-ion reversed the relevant metal-defence-mutant hypersensitivity to allicin in yeast.","subject":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"object":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"8469972f-a08d-55a4-857c-c0f9e376ce1b","stable_key":"c836a883-ac18-5eb2-9971-2f0b542feba8:yeast-metal-rescue-zinc-ion-event","event_type":"experimental_observation","label":"Added zinc-ion reversed the relevant metal-defence-mutant hypersensitivity to allicin in yeast.","description":"**Yeast metal requirements.** In a chemical-genetic screen of Saccharomyces cerevisiae, loss of CTR1, MAC1, or ZAP1 increased sensitivity to allicin; copper or zinc additions rescued the relevant sensitized phenotypes. This supports involvement of metal acquisition or metalloprotein function but does not discriminate those alternatives. It is not direct proof that allicin binds each protein, chelates all intracellular copper, or causes human mineral deficiency. Exact supplementation concentrations and all strain-specific contrasts remain unextracted from the accessible abstract. [Prescott and Panaretou 2017](https://pubmed.ncbi.nlm.nih.gov/28421744/)","status":"provisional","compartment":null,"participants":[{"entity":{"id":"49c806c2-7041-5020-8b3b-fa04ffa122ac","slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"},"role":"tested factor","stoichiometry":null,"state_label":"Metal addition to the corresponding sensitized allicin-exposed yeast","sequence_order":0,"notes":""},{"entity":{"id":"29ccc26a-b3de-57b2-b46d-5f6206054867","slug":"yeast-allicin-growth","display_name":"Saccharomyces cerevisiae growth during allicin exposure","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"85c86fcf-3060-5fae-b567-4f089990ab2d","slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"},"role":"joint exposure","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, exact concentrations or species-specific attribution remain unextracted unless explicitly stated in the abstract or separately verified publisher text.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"present","comparator":"Same sensitized yeast with allicin without added metal","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"allicin","display_name":"Allicin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"added in relevant mutant background","comparator":"Same sensitized yeast with allicin without added metal","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"zinc-ion","display_name":"Zinc(II) ion","entity_type_key":"ion"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Metal addition to the corresponding sensitized allicin-exposed yeast\", \"comparator\": \"Same sensitized yeast with allicin without added metal\", \"endpoint\": \"Added zinc-ion reversed the relevant metal-defence-mutant hypersensitivity to allicin in yeast.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"zinc-ion\", \"state\": \"added in relevant mutant background\"}, {\"entity_slug\": \"allicin\", \"state\": \"present\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"Saccharomyces cerevisiae sensitized-mutant rescue; accessible abstract; exact individual strain-dose mapping not fully extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Abstract reports copper/zinc rescue but does not provide all strain-specific values. No human mineral-deficiency or supplementation claim.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Added zinc-ion reversed the relevant metal-defence-mutant hypersensitivity to allicin in yeast.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A Mini HIP HOP Assay Uncovers a Central Role for Copper and Zinc in the Antifungal Mode of Action of Allicin. | 2017 | DOI 10.1021/acs.jafc.7b00250 | PMID 28421744 | https://pubmed.ncbi.nlm.nih.gov/28421744/ | https://doi.org/10.1021/acs.jafc.7b00250","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 59-59; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"43073a0c-246c-5531-90be-b74d1f4be40c","evidence_kind":"source_excerpt","locator":"Lines 59-59","start_line":59,"end_line":59,"excerpt":"**Yeast metal requirements.** In a chemical-genetic screen of Saccharomyces cerevisiae, loss of CTR1, MAC1, or ZAP1 increased sensitivity to allicin; copper or zinc additions rescued the relevant sensitized phenotypes. This supports involvement of metal acquisition or metalloprotein function but does not discriminate those alternatives. It is not direct proof that allicin binds each protein, chelates all intracellular copper, or causes human mineral deficiency. Exact supplementation concentrations and all strain-specific contrasts remain unextracted from the accessible abstract. [Prescott and Panaretou 2017](https://pubmed.ncbi.nlm.nih.gov/28421744/)","model_system":"Saccharomyces cerevisiae sensitized-mutant rescue; accessible abstract; exact individual strain-dose mapping not fully extracted.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"10820a6b-594a-547d-97f9-906ab4cc1d6e","stable_key":"import-c836a883-ac18-5eb2-9971-2f0b542feba8","title":"Allicin: detailed mechanisms of action (reviewed 5 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"2475d3eb681100a0a34577a47b7cba5b251df866fbd6ce795122ccabee360018","revision_id":"590df96d-2ed1-5763-b04c-bf0e096e603c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}