{"id":"e07faf93-4188-549d-82ba-99b98de94f63","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-apt2-palmitoylation","predicate":"has_contextual_experimental_effect","statement":"Sulforaphane reduced Alk14 labeling of immunoprecipitated APT2 in transfected HEK293 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"a3910d86-8116-5d44-8ad3-7c8886b3a01b","mechanism_event_label":"sfn apt2 palmitoylation","subject":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"object":{"id":"70d202f2-51b8-5db4-b3a4-1aee612569f2","slug":"human-apt2-palmitoylation","display_name":"Human APT2 palmitoylation readout","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"a3910d86-8116-5d44-8ad3-7c8886b3a01b","stable_key":"e054f0f5-417f-5850-a874-91ff2c9de631:sfn-apt2-palmitoylation","event_type":"experimental_result","label":"sfn apt2 palmitoylation","description":"Sulforaphane reduced Alk14 labeling of immunoprecipitated APT2 in transfected HEK293 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"67e70215-70c4-546a-84b4-0819fac0b326","slug":"sulforaphane","display_name":"Sulforaphane / SFN, stereochemistry specified per study","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"70d202f2-51b8-5db4-b3a4-1aee612569f2","slug":"human-apt2-palmitoylation","display_name":"Human APT2 palmitoylation readout","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f2ffef5b-5457-50a8-b787-b8f24430f610","slug":"lypla2","display_name":"LYPLA2","entity_type_key":"protein"},"role":"measured protein","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_cache","value_text":"artifacts/discovery-research/round6-sources/sulforaphane-apt2.pdf; SHA256 31e2993f88fe05eec199b9013173b29379b3346b9696ac766771173ddd0f181a","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HEK293 expressing FLAG-tagged human APT2","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"100 micromolar sulforaphane for 3 hours followed by 50 micromolar Alk14 for 5 hours; hydroxylamine control; three separate experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. The authors could not distinguish reduced palmitoylation from enhanced depalmitoylation. This assay does not measure GPX4 or ZDHHC6.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"Human experimental cells; construct species unresolved where stated for discussion-only nulls","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_locator","value_text":"Figure 4B; Methods 2.8","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"https://doi.org/10.1016/j.bbrc.2024.150244","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_access","value_text":"Full six-page primary PDF, methods and discussion read; Figure 4 visually inspected. No raw-data reanalysis.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5ecb8f19-c634-5123-83dd-611e2ce6d057","evidence_kind":"source_excerpt","locator":"Lines 19-27","start_line":19,"end_line":27,"excerpt":"Sulforaphane reduced Alk14 labeling of immunoprecipitated APT2 in transfected HEK293 cells.\nprimary_references: https://doi.org/10.1016/j.bbrc.2024.150244\nprimary_locator: Figure 4B; Methods 2.8\nsource_access: Full six-page primary PDF, methods and discussion read; Figure 4 visually inspected. No raw-data reanalysis.\nevidence_cache: artifacts/discovery-research/round6-sources/sulforaphane-apt2.pdf; SHA256 31e2993f88fe05eec199b9013173b29379b3346b9696ac766771173ddd0f181a\nexperimental_model: Human HEK293 expressing FLAG-tagged human APT2\norganism: Human experimental cells; construct species unresolved where stated for discussion-only nulls\nexposure: 100 micromolar sulforaphane for 3 hours followed by 50 micromolar Alk14 for 5 hours; hydroxylamine control; three separate experiments.\nlimitations: These are separate experimental observations, not a demonstrated sulforaphane-GPX4-ZDHHC6 pathway. Sulforaphane-driven APT2 relocalization is not equivalent to genetic depletion or general catalytic inhibition. No dietary, clinical or selenium-repletion effect is inferred. Protein stability, palmitoylation and substrate output remain different measurements. The authors could not distinguish reduced palmitoylation from enhanced depalmitoylation. This assay does not measure GPX4 or ZDHHC6.","model_system":"Human HEK293 expressing FLAG-tagged human APT2","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curator paraphrase and primary locator; not a verbatim publisher quote. Null-result limitations retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d7785cbf-e87c-5662-a76c-7ef11586cca5","stable_key":"import-e054f0f5-417f-5850-a874-91ff2c9de631","title":"APT2: sulforaphane engagement, GPX4 stability and ZDHHC6 turnover","document_type":"imported_text","citation_label":"Targeted primary-literature curation from recursive ZDHHC6 exploration, 2026-09-20.","file_path":"","sha256":"011acbeafd419a6826789806b59b5d7ff312b222762824580036325083586956","revision_id":"663a0bd1-3e46-5fee-ab0f-dcf992b2a809","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}