{"id":"e07926d4-b4b0-570d-81b5-86f0f578d668","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mat2a-prmt5-vulnerability","predicate":"supports","statement":"MAT2A depletion preferentially reduced growth and PRMT5 methylation activity in MTAP-deleted cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"bfc9f530-a5dc-5a50-beb7-6b35784b9c3a","mechanism_event_label":"Loss of a recycling enzyme can make cells more sensitive to reduced donor synthesis.","subject":{"id":"bbc04455-e242-5f4d-9a6c-5b2ee6a6cb41","slug":"human-mat2a","display_name":"Human methionine adenosyltransferase 2A / MAT2A","entity_type_key":"protein"},"object":{"id":"4f3fefcc-85f6-5420-9931-195aa5022834","slug":"prmt5","display_name":"Human protein arginine methyltransferase 5 / PRMT5","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"bfc9f530-a5dc-5a50-beb7-6b35784b9c3a","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mat2a-prmt5-vulnerability-event","event_type":"observed_relationship","label":"Loss of a recycling enzyme can make cells more sensitive to reduced donor synthesis.","description":"MAT2A depletion preferentially reduced growth and PRMT5 methylation activity in MTAP-deleted cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bbc04455-e242-5f4d-9a6c-5b2ee6a6cb41","slug":"human-mat2a","display_name":"Human methionine adenosyltransferase 2A / MAT2A","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4f3fefcc-85f6-5420-9931-195aa5022834","slug":"prmt5","display_name":"Human protein arginine methyltransferase 5 / PRMT5","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"825f2da2-01bc-5874-a3c6-64f5ac867db5","slug":"s-adenosylmethionine","display_name":"S-Adenosyl-L-methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"fcd3b947-abdf-5e52-904f-a24f475e446c","slug":"mtap","display_name":"Human methylthioadenosine phosphorylase / MTAP","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"525860da-a986-5d19-97f0-a257a454b649","slug":"5-methylthioadenosine","display_name":"5′-Methylthioadenosine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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