{"id":"e04dfc4a-fd39-57df-b78a-17d0472255c7","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-gpt2-reaction","predicate":"catalyzes-transamination-of","statement":"Recombinant human GPT2 catalyzed alanine transamination, confirming a second human alanine aminotransferase distinct from GPT.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"b6301f03-ea25-5a83-aab1-26e0d54d0c26","mechanism_event_label":"Human alanine metabolism has a separately encoded second enzyme.","subject":{"id":"59baef49-fed8-5b6f-90ca-b8efdf021608","slug":"gpt2","display_name":"Human mitochondrial alanine aminotransferase / GPT2","entity_type_key":"protein"},"object":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"b6301f03-ea25-5a83-aab1-26e0d54d0c26","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-gpt2-reaction-event","event_type":"biochemical_relationship","label":"Human alanine metabolism has a separately encoded second enzyme.","description":"Recombinant human GPT2 catalyzed alanine transamination, confirming a second human alanine aminotransferase distinct from GPT.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"59baef49-fed8-5b6f-90ca-b8efdf021608","slug":"gpt2","display_name":"Human mitochondrial alanine aminotransferase / GPT2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"628ddc2d-e4d9-59c7-ae76-0c0cab80a6b1","slug":"2-oxoglutarate","display_name":"2-Oxoglutarate","entity_type_key":"small_molecule"},"role":"amino acceptor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"91e6d4f5-fba1-542d-b68a-57cc28e1e425","slug":"pyruvate","display_name":"Pyruvate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"product","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human GPT2 expressed in E. coli","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Functional-expression evidence; this study did not quantify B6-deficiency sensitivity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Human alanine metabolism has a separately encoded second enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-gpt2-2002] cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase (2002). https://pubmed.ncbi.nlm.nih.gov/11863375/ DOI: 10.1006/geno.2002.6722","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"4b94ac84-b9e3-5f18-9177-ff8e517ae36d","evidence_kind":"source_excerpt","locator":"Lines 776-785","start_line":776,"end_line":785,"excerpt":"### b6-met-gpt2-reaction\nRecombinant human GPT2 catalyzed alanine transamination, confirming a second human alanine aminotransferase distinct from GPT.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Human alanine metabolism has a separately encoded second enzyme.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Human GPT2 expressed in E. coli\nlimitations: Functional-expression evidence; this study did not quantify B6-deficiency sensitivity.\n[b6-gpt2-2002] cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase (2002). https://pubmed.ncbi.nlm.nih.gov/11863375/ DOI: 10.1006/geno.2002.6722","model_system":"Human GPT2 expressed in E. coli","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-gpt2-2002] cDNA cloning, genomic structure, chromosomal mapping, and functional expression of a novel human alanine aminotransferase (2002). https://pubmed.ncbi.nlm.nih.gov/11863375/ DOI: 10.1006/geno.2002.6722","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}