{"id":"dfe63ed5-1939-5080-bb5d-4ae70e58fa37","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nlrp3-loss","predicate":"required_for","statement":"Nlrp3-deficient macrophages failed to mount the normal silica-induced caspase-1/IL-1 beta response.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"5e490dc0-7bff-5c93-964c-f3d7a1c99536","mechanism_event_label":"The sensor is a distinct necessary component in this model.","subject":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"object":{"id":"314cd2c5-bd37-51fb-bff8-cee2fccf6997","slug":"mouse-silica-caspase1-activation","display_name":"Caspase-1 activation after silica in mouse macrophages","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5e490dc0-7bff-5c93-964c-f3d7a1c99536","stable_key":"3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd:silica-nlrp3-loss-event","event_type":"observed_relationship","label":"The sensor is a distinct necessary component in this model.","description":"Nlrp3-deficient macrophages failed to mount the normal silica-induced caspase-1/IL-1 beta response.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae25f14e-2a54-5094-9952-a97b718f94a3","slug":"mouse-nlrp3","display_name":"Mouse NLRP3","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"314cd2c5-bd37-51fb-bff8-cee2fccf6997","slug":"mouse-silica-caspase1-activation","display_name":"Caspase-1 activation after silica in mouse macrophages","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"11b1cdb5-1b10-586b-9917-b4da18ac072f","slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"b2d4231b-3893-5d4e-8b52-d0effdaa26c9","slug":"crystalline-silica","display_name":"Crystalline silica / quartz particles","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"69b5a136-93e3-567b-a97a-d33853229c81","slug":"mouse-casp1","display_name":"Mouse caspase-1","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"c17455e2-5805-55e7-9ecc-09c953115c84","slug":"mouse-il1b","display_name":"Mouse IL-1 beta","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"LPS-primed mouse macrophage genetic comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Priming and activation are separate; dietary silicon is not the particle stimulus. Correction record: A 2026 author correction replaces an erroneously duplicated lower-left Fig. 8c micrograph with the retrieved original. This is a figure correction, not a scientific contradiction or retraction. https://www.nature.com/articles/s41590-026-02468-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Silica collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"silica","display_name":"Silica and soluble silicon","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The sensor is a distinct necessary component in this model.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Silica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18604214/ · DOI 10.1038/ni.1631","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"269ab788-5191-5a3f-abf3-73e980fabf7b","evidence_kind":"source_excerpt","locator":"Lines 440-446","start_line":440,"end_line":446,"excerpt":"## silica-nlrp3-loss\nThe sensor is a distinct necessary component in this model.\nNlrp3-deficient macrophages failed to mount the normal silica-induced caspase-1/IL-1 beta response.\nModel: LPS-primed mouse macrophage genetic comparison.\nLimitations: Priming and activation are separate; dietary silicon is not the particle stimulus. Correction record: A 2026 author correction replaces an erroneously duplicated lower-left Fig. 8c micrograph with the retrieved original. This is a figure correction, not a scientific contradiction or retraction. https://www.nature.com/articles/s41590-026-02468-9\nEvidence access: Primary full text\nSilica crystals and aluminum salts activate the NALP3 inflammasome through phagosomal destabilization. · 2008 · https://pubmed.ncbi.nlm.nih.gov/18604214/ · DOI 10.1038/ni.1631","model_system":"LPS-primed mouse macrophage genetic comparison.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc4b461e-dd46-57ab-8213-8aeb7ff9e8d0","stable_key":"import-3b71c9a2-e2fe-5ab8-a85b-15ae1be836dd","title":"Silica: soluble silicon, cellular transport and particle-specific mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"e05d81962a1ca6cbfc68fce4fd3dbcdea67c8e61332c19f4fec5d0c3ab8bf1b9","revision_id":"6433ed49-a331-571b-a611-21e56c3ce4bc","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}