{"id":"dfe33a48-367b-594f-ab04-5b0126139a44","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-copper-carrier","predicate":"inhibits","statement":"Copper inhibited rat-liver CACT carnitine exchange in proteoliposomes, with a native-protein IC50 of 1.6 micromolar.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"54d60675-1217-537d-80a9-058c9c86661d","mechanism_event_label":"Metal exposure can interfere with the carrier in a controlled assay.","subject":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"object":{"id":"ee012f58-8529-5dab-a512-5236fb2b3d50","slug":"rat-cact-transport-activity","display_name":"Rat mitochondrial CACT transport activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"54d60675-1217-537d-80a9-058c9c86661d","stable_key":"6cdb37aa-8998-5ea8-829d-4f995caf98fc:l-carnitine-copper-carrier-event","event_type":"observed_relationship","label":"Metal exposure can interfere with the carrier in a controlled assay.","description":"Copper inhibited rat-liver CACT carnitine exchange in proteoliposomes, with a native-protein IC50 of 1.6 micromolar.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2a870bb5-05a0-5e51-a70c-a9a843a8b571","slug":"copper-ii","display_name":"Copper(II) ion","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ee012f58-8529-5dab-a512-5236fb2b3d50","slug":"rat-cact-transport-activity","display_name":"Rat mitochondrial CACT transport activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"49164668-8ef7-5bde-afda-1c6aadd87c68","slug":"rat-slc25a20","display_name":"Rat carnitine-acylcarnitine translocase / Slc25a20","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"6e34c035-9371-578f-b799-fd2a14e9e40f","slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Native rat-liver carrier; recombinant cysteine mutants supported involvement of C136 and C155.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Free copper in an assay is not dietary copper exposure; no clinical deficiency or toxicity threshold follows.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Carnitine collection; isomer, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-carnitine","display_name":"L-Carnitine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Metal exposure can interfere with the carrier in a controlled assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Effect of Copper on the Mitochondrial Carnitine/Acylcarnitine Carrier Via Interaction with Cys136 and Cys155. Possible Implications in Pathophysiology. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32070004/ · DOI 10.3390/molecules25040820","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"c0631740-e67f-5235-bcee-170018cc641a","evidence_kind":"source_excerpt","locator":"Lines 138-144","start_line":138,"end_line":144,"excerpt":"## l-carnitine-copper-carrier\nMetal exposure can interfere with the carrier in a controlled assay.\nCopper inhibited rat-liver CACT carnitine exchange in proteoliposomes, with a native-protein IC50 of 1.6 micromolar.\nModel: Native rat-liver carrier; recombinant cysteine mutants supported involvement of C136 and C155.\nLimitations: Free copper in an assay is not dietary copper exposure; no clinical deficiency or toxicity threshold follows.\nEvidence access: Primary abstract\nEffect of Copper on the Mitochondrial Carnitine/Acylcarnitine Carrier Via Interaction with Cys136 and Cys155. Possible Implications in Pathophysiology. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32070004/ · DOI 10.3390/molecules25040820","model_system":"Native rat-liver carrier; recombinant cysteine mutants supported involvement of C136 and C155.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e2c9c42b-806a-55e2-abde-b0320ea94704","stable_key":"import-6cdb37aa-8998-5ea8-829d-4f995caf98fc","title":"L-Carnitine: synthesis, acyl-group transport, fuel selection and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"6fcd86d9d02babb48b2ce658d506afe84580ad4b242f93f11f742d934fa5b361","revision_id":"398e3b52-45bc-52e3-891e-656c19ca674f","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}