{"id":"dfd33926-f19d-5da6-b22c-ad1461911346","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-oat3","predicate":"transports","statement":"Steviol trans-stimulated tracer efflux through human OAT3 expressed in Xenopus oocytes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"caa0703b-68ef-5c37-95f8-edc164842295","mechanism_event_label":"A second renal organic-anion transporter handles the same metabolite.","subject":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"object":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"caa0703b-68ef-5c37-95f8-edc164842295","stable_key":"6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7:stevia-oat3-event","event_type":"observed_relationship","label":"A second renal organic-anion transporter handles the same metabolite.","description":"Steviol trans-stimulated tracer efflux through human OAT3 expressed in Xenopus oocytes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4606352d-ad2e-580f-87eb-e0ccf0d02fd9","slug":"slc22a8","display_name":"Human organic anion transporter 3 / SLC22A8","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8ba32fe6-183f-5cd5-b386-02e6440e78a0","slug":"steviol","display_name":"Steviol","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c78086c3-7657-589b-b353-1691df2d9f8f","slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cloned transporter in frog oocytes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"OAT3 and OATP transporters are different families and are not merged here.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Stevia collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"stevia","display_name":"Stevia","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A second renal organic-anion transporter handles the same metabolite.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Transport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"transport_effect","value_text":"depends","comparator":null,"unit":null,"notes":"Inferred from trans-stimulation of a tracer's efflux, which shows movement without fixing its direction.","entity":null},{"dimension":"transport_pool","value_text":"the cytosol across the basolateral membrane","comparator":null,"unit":null,"notes":"Inferred from trans-stimulation of a tracer's efflux, which shows movement without fixing its direction.","entity":null}],"evidence":[{"id":"c7317f35-2b60-51bf-b73b-927d9f6122ed","evidence_kind":"source_excerpt","locator":"Lines 106-112","start_line":106,"end_line":112,"excerpt":"## stevia-oat3\nA second renal organic-anion transporter handles the same metabolite.\nSteviol trans-stimulated tracer efflux through human OAT3 expressed in Xenopus oocytes.\nModel: Human cloned transporter in frog oocytes.\nLimitations: OAT3 and OATP transporters are different families and are not merged here.\nEvidence access: Primary abstract\nTransport of the natural sweetener stevioside and its aglycone steviol by human organic anion transporter (hOAT1; SLC22A6) and hOAT3 (SLC22A8). · 2005 · https://pubmed.ncbi.nlm.nih.gov/15644426/ · DOI 10.1124/jpet.104.080366","model_system":"Human cloned transporter in frog oocytes.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"295304c9-609c-5b05-a18f-a00ab7b965ed","stable_key":"import-6b15b2de-9ce9-5b6f-ab8b-3c7dbb4c15c7","title":"Stevia: glycoside metabolism, taste, ion channels and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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