{"id":"dfcf6d35-6455-57eb-b1ea-db463494ab71","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-nad-ratio","predicate":"increases_in_recorded_comparison","statement":"Apigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"7e91aa62-2775-57e9-8725-f3174b9ac073","mechanism_event_label":"Apigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"6fe789f0-efb9-5d2c-b43a-9d705a5da004","slug":"cellular-nad-ratio","display_name":"Cellular NAD+/NADH ratio","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"7e91aa62-2775-57e9-8725-f3174b9ac073","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-gout-nad-ratio-event","event_type":"experimental_observation","label":"Apigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.","description":"### api-gout-nad-ratio\n\nApigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.\nA higher ratio does not itself demonstrate more NAD+ molecules.\nModel and exposure: Mouse BMDMs; separate NAD+ and NADH assay readouts.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Apigenin plus MSU","sequence_order":0,"notes":""},{"entity":{"id":"6fe789f0-efb9-5d2c-b43a-9d705a5da004","slug":"cellular-nad-ratio","display_name":"Cellular NAD+/NADH ratio","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"283ed24b-06a1-50aa-9281-df3bac6ce37e","slug":"nad-plus","display_name":"NAD+","entity_type_key":"small_molecule"},"role":"ratio numerator","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"9fbf2e03-16a9-5ca7-965c-0bfc07ca24da","slug":"nadh","display_name":"NADH","entity_type_key":"small_molecule"},"role":"decreased denominator","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"Apigenin plus MSU","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"0.2 mg/mL MSU challenge","comparator":"MSU alone","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"monosodium-urate-crystals","display_name":"Monosodium urate crystals","entity_type_key":"small_molecule"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Apigenin plus MSU\", \"comparator\": \"MSU alone\", \"endpoint\": \"Apigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"apigenin\", \"state\": \"Apigenin plus MSU\"}, {\"entity_slug\": \"monosodium-urate-crystals\", \"state\": \"0.2 mg/mL MSU challenge\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Mouse BMDMs; separate NAD+ and NADH assay readouts.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Apigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-gout-nad-ratio; A higher ratio does not itself demonstrate more NAD+ molecules.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"739d216a-5a81-526f-96a7-e52915b2b24d","evidence_kind":"source_excerpt","locator":"Lines 308-314","start_line":308,"end_line":314,"excerpt":"### api-gout-nad-ratio\n\nApigenin increased the NAD+/NADH ratio in the MSU experiment mainly by reducing the MSU-associated NADH increase.\nA higher ratio does not itself demonstrate more NAD+ molecules.\nModel and exposure: Mouse BMDMs; separate NAD+ and NADH assay readouts.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: The NADase CD38 is a central regulator in gouty inflammation and a novel druggable therapeutic target. | 2024 | https://pubmed.ncbi.nlm.nih.gov/38493256/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC11058052/ | https://doi.org/10.1007/s00011-024-01863-y | Access: Primary full text: relevant Results/Methods and figure descriptions reviewed; supplements only where explicitly stated.","model_system":"Mouse BMDMs; separate NAD+ and NADH assay readouts.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}