{"id":"dfcc7085-6b67-5351-b935-41c56f279599","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-differentiation","predicate":"increases_in_recorded_comparison","statement":"The combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"ecedecdf-dee1-5bf3-b5ce-e4bb43b0408c","mechanism_event_label":"The combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.","subject":{"id":"6d70addf-e302-5605-9c6a-02358209c876","slug":"nmn-apigenin-combination-2026","display_name":"NMN plus apigenin preparation in the 2026 musculoskeletal study","entity_type_key":"chemical_species"},"object":{"id":"345badc3-6b80-55f5-ae2d-0d94cb6b9548","slug":"api-nmn-trilineage-differentiation","display_name":"Chondrogenic, osteogenic and myogenic readouts in the 2026 combination study","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"ecedecdf-dee1-5bf3-b5ce-e4bb43b0408c","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-nmn-differentiation-event","event_type":"experimental_observation","label":"The combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.","description":"### api-nmn-differentiation\n\nThe combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.\nThese are model-specific readouts, not proof that apigenin increases systemic calcium or cures human musculoskeletal disease.\nModel and exposure: SOX9/COL2A1, ALP/calcium nodules/RUNX2/SP7/OCN, and MyoD1/MYOG/myotube endpoints.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | 2026 | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468 | Access: Primary main-text Results and figure descriptions reviewed. Detailed supporting Methods/supplements inaccessible; exact doses and preparation details not independently extracted.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6d70addf-e302-5605-9c6a-02358209c876","slug":"nmn-apigenin-combination-2026","display_name":"NMN plus apigenin preparation in the 2026 musculoskeletal study","entity_type_key":"chemical_species"},"role":"tested factor","stoichiometry":null,"state_label":"NMN plus apigenin","sequence_order":0,"notes":""},{"entity":{"id":"345badc3-6b80-55f5-ae2d-0d94cb6b9548","slug":"api-nmn-trilineage-differentiation","display_name":"Chondrogenic, osteogenic and myogenic readouts in the 2026 combination study","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"8e2a5a1f-cd49-5f28-8611-325266fb78a6","slug":"calcium","display_name":"Calcium","entity_type_key":"nutrient_element"},"role":"mineralization readout, not serum nutrient level","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary main-text Results and figure descriptions reviewed. Detailed supporting Methods/supplements inaccessible; exact doses and preparation details not independently extracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_condition","value_text":"co-administered","comparator":"Stressed differentiation controls","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"co-administered","comparator":"Stressed differentiation controls","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"nmn","display_name":"NMN","entity_type_key":"small_molecule"}},{"dimension":"experimental_condition","value_text":"NMN plus apigenin","comparator":"Stressed differentiation controls","unit":null,"notes":"Condition belongs to the full experimental contrast; do not separate a joint intervention.","entity":{"slug":"nmn-apigenin-combination-2026","display_name":"NMN plus apigenin preparation in the 2026 musculoskeletal study","entity_type_key":"chemical_species"}},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"NMN plus apigenin\", \"comparator\": \"Stressed differentiation controls\", \"endpoint\": \"The combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.\", \"effect_direction\": \"increase\", \"combination\": \"joint\", \"conditions\": [{\"entity_slug\": \"nmn-apigenin-combination-2026\", \"state\": \"NMN plus apigenin\"}, {\"entity_slug\": \"nmn\", \"state\": \"co-administered\"}, {\"entity_slug\": \"apigenin\", \"state\": \"co-administered\"}]}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"SOX9/COL2A1, ALP/calcium nodules/RUNX2/SP7/OCN, and MyoD1/MYOG/myotube endpoints.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-nmn-differentiation; These are model-specific readouts, not proof that apigenin increases systemic calcium or cures human musculoskeletal disease.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"58068961-7afd-5a1f-830c-313613329ec8","evidence_kind":"source_excerpt","locator":"Lines 540-546","start_line":540,"end_line":546,"excerpt":"### api-nmn-differentiation\n\nThe combined preparation improved chondrogenic matrix, osteogenic mineralization and myogenic differentiation readouts in the reported stress models.\nThese are model-specific readouts, not proof that apigenin increases systemic calcium or cures human musculoskeletal disease.\nModel and exposure: SOX9/COL2A1, ALP/calcium nodules/RUNX2/SP7/OCN, and MyoD1/MYOG/myotube endpoints.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Double-Pronged NAD Preservation: Delaying Cellular Senescence and Initiating Musculoskeletal Regeneration. | 2026 | https://pubmed.ncbi.nlm.nih.gov/41944220/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC13054677/ | https://doi.org/10.1111/acel.70468 | Access: Primary main-text Results and figure descriptions reviewed. Detailed supporting Methods/supplements inaccessible; exact doses and preparation details not independently extracted.","model_system":"SOX9/COL2A1, ALP/calcium nodules/RUNX2/SP7/OCN, and MyoD1/MYOG/myotube endpoints.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}