{"id":"df983ff0-8391-55f6-a2c2-e8b5dfd312a8","stable_key":"research:selenoo-loss-increases-complex-ii-activity","predicate":"loss-increases-activity","statement":"SELENOO deletion increased complex-II activity in the tested melanoma model.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"literature_reviewed:direct_experimental","direction":"positive","is_public":true,"mechanism_event_id":"034df6e8-9b04-5c61-b481-b46fa2365fa1","mechanism_event_label":"Removing SELENOO increased respiratory complex-II activity in this model.","subject":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"object":{"id":"4dc2faa3-f38c-5813-b7f2-fe19a0ab7bfa","slug":"respiratory-complex-ii","display_name":"Respiratory complex II","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"034df6e8-9b04-5c61-b481-b46fa2365fa1","stable_key":"research:selenoo-loss-increases-complex-ii-activity","event_type":"genetic-perturbation","label":"Removing SELENOO increased respiratory complex-II activity in this model.","description":"SELENOO deletion increased complex-II activity in the tested melanoma model.","status":"active","compartment":{"slug":"mitochondria","display_name":"Mitochondria"},"participants":[{"entity":{"id":"90e4a389-1062-5489-bae1-d49f337c98f0","slug":"selenoo","display_name":"SELENOO","entity_type_key":"protein"},"role":"perturbed-protein","stoichiometry":null,"state_label":"deleted","sequence_order":0,"notes":""},{"entity":{"id":"4dc2faa3-f38c-5813-b7f2-fe19a0ab7bfa","slug":"respiratory-complex-ii","display_name":"Respiratory complex II","entity_type_key":"protein_complex"},"role":"measured-complex","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"dbebfa19-a142-5d23-b8dd-e209c2dd78d5","slug":"sdha","display_name":"SDHA","entity_type_key":"protein"},"role":"complex-subunit","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cell_type","value_text":"melanoma","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Selenoo deletion","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Does not isolate SDHA AMPylation as the sole cause.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"organism","value_text":"mouse","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"cb436320-b39d-584c-9022-5cfc0c09d8c8","evidence_kind":"curated_literature_summary","locator":"lines 474-484","start_line":474,"end_line":484,"excerpt":"## selenoo-loss-increases-complex-ii-activity\n\nRemoving SELENOO increased respiratory complex-II activity in this model.\n\nSELENOO deletion increased complex-II activity in the tested melanoma model.\n\nOrganism: mouse\nCell type: melanoma\nExperimental model: Selenoo deletion\nLimitations: Does not isolate SDHA AMPylation as the sole cause.\nPrimary reference: [Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity](https://pubmed.ncbi.nlm.nih.gov/39700395/)","model_system":"Selenoo deletion","directness":"author_interpretation","verification_status":"secondary_verified","notes":"Curated summary; inspect the linked primary papers for original methods and results.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4f892f13-06ea-5199-a33c-a703f35c80ae","stable_key":"selenium-research-2026-09-17","title":"Selenium: literature corrections and mechanism additions","document_type":"curated_literature_review","citation_label":"Metabolic Ledger literature curation, 17 September 2026; primary papers linked individually","file_path":"","sha256":"0b818b10c1c7120e5caf7f4d4019d7bd025d745692e424f515d3ef903c9ab7f3","revision_id":"80984e03-5f0f-5877-8094-afef7637444e","review_status":"secondary_verified","notes":"Secondary curated summaries of primary experiments, with explicit models and limitations. Not archived primary full text."}}],"relations":[],"conflicts":[],"corrections":[],"research":{"topic":"mitochondrial-metabolism","plain_language":"Removing SELENOO increased respiratory complex-II activity in this model.","evidence_scope":"direct_experimental","papers":[{"key":"melo-2025-selenoo-sdha","title":"Selenoprotein O Promotes Melanoma Metastasis and Regulates Mitochondrial Complex II Activity","url":"https://pubmed.ncbi.nlm.nih.gov/39700395/","doi":"10.1158/0008-5472.CAN-23-2194","year":2025,"model":"Biochemical assays and mouse melanoma models","summary":"Identifies SDHA AMPylation and altered complex-II activity after SELENOO loss in melanoma experiments."}]}}