{"id":"df89022b-a2c2-543d-8a6f-2465097a3608","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tcell-function","predicate":"improves","statement":"Spermidine improved effector-function readouts in cultured T cells from older donors.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"af0a0293-6e86-53f4-9bc0-072c58c5c0d8","mechanism_event_label":"Functional measurements accompanied the recycling markers.","subject":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"object":{"id":"e4518f2e-4a6b-564c-b608-2d8e0c1a5d3e","slug":"human-aged-tcell-effector-response","display_name":"Human older-donor T-cell effector response ex vivo","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"af0a0293-6e86-53f4-9bc0-072c58c5c0d8","stable_key":"88eb7407-f147-558b-889b-0c89c4e0aa4a:spermidine-tcell-function-event","event_type":"observed_relationship","label":"Functional measurements accompanied the recycling markers.","description":"Spermidine improved effector-function readouts in cultured T cells from older donors.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"7014993c-468f-5c6b-ab04-80c87f9dfd9f","slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"e4518f2e-4a6b-564c-b608-2d8e0c1a5d3e","slug":"human-aged-tcell-effector-response","display_name":"Human older-donor T-cell effector response ex vivo","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary human cells; cytokine and effector-protein assays.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The vaccine cohort provided associations; subjects were not randomized to oral spermidine for vaccine enhancement.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Spermidine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"spermidine","display_name":"Spermidine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Functional measurements accompanied the recycling markers.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Autophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33317695/ · DOI 10.7554/eLife.57950","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"75dd4136-ee5b-5afd-9db8-32ddd8e0b3ad","evidence_kind":"source_excerpt","locator":"Lines 254-260","start_line":254,"end_line":260,"excerpt":"## spermidine-tcell-function\nFunctional measurements accompanied the recycling markers.\nSpermidine improved effector-function readouts in cultured T cells from older donors.\nModel: Primary human cells; cytokine and effector-protein assays.\nLimitations: The vaccine cohort provided associations; subjects were not randomized to oral spermidine for vaccine enhancement.\nEvidence access: Primary full text\nAutophagy in T cells from aged donors is maintained by spermidine and correlates with function and vaccine responses. · 2020 · https://pubmed.ncbi.nlm.nih.gov/33317695/ · DOI 10.7554/eLife.57950","model_system":"Primary human cells; cytokine and effector-protein assays.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4348f3f8-0180-58a3-b61c-55d03bb322d2","stable_key":"import-88eb7407-f147-558b-889b-0c89c4e0aa4a","title":"Spermidine: biosynthesis, hypusination, transport and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"8eec8784bc2bd0384cfd53c6603c4ced8e53c227d9ab29ed85594c43e34c68ef","revision_id":"7c421a18-4526-59f6-b84e-4be533bc35ac","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}