{"id":"df3c27cb-9fa1-5020-be06-a9df68632812","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-neutrophil-release","predicate":"hydrolyzes_to","statement":"Supernatants from activated human neutrophils converted luteolin monoglucuronide to free luteolin, consistent with released beta-glucuronidase.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fba97508-4232-53a1-a6fe-f4941ea9a498","mechanism_event_label":"Inflamed cells can locally regenerate the unconjugated molecule.","subject":{"id":"54cf9dcd-f680-586f-95d9-8f7192bb591b","slug":"gusb","display_name":"Human beta-glucuronidase / GUSB","entity_type_key":"protein"},"object":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fba97508-4232-53a1-a6fe-f4941ea9a498","stable_key":"22527305-af1f-536a-b419-7e73f08cc3ec:luteolin-neutrophil-release-event","event_type":"observed_relationship","label":"Inflamed cells can locally regenerate the unconjugated molecule.","description":"Supernatants from activated human neutrophils converted luteolin monoglucuronide to free luteolin, consistent with released beta-glucuronidase.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"54cf9dcd-f680-586f-95d9-8f7192bb591b","slug":"gusb","display_name":"Human beta-glucuronidase / GUSB","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"80472c4b-ecc2-52e3-a766-f8463294774f","slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"f0186989-65b0-5f7a-95d9-d25a7789222b","slug":"luteolin-monoglucuronides","display_name":"Luteolin monoglucuronides, position unresolved in study","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Ionomycin/cytochalasin-B-stimulated human neutrophils; HPLC.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Position of the monoglucuronide is not resolved in the abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Luteolin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"luteolin","display_name":"Luteolin / 3′,4′,5,7-tetrahydroxyflavone","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Inflamed cells can locally regenerate the unconjugated molecule.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Deglucuronidation of a flavonoid, luteolin monoglucuronide, during inflammation. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11717168/","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"91d0269e-6ae1-54be-9a27-e003178606f4","evidence_kind":"source_excerpt","locator":"Lines 156-162","start_line":156,"end_line":162,"excerpt":"## luteolin-neutrophil-release\nInflamed cells can locally regenerate the unconjugated molecule.\nSupernatants from activated human neutrophils converted luteolin monoglucuronide to free luteolin, consistent with released beta-glucuronidase.\nModel: Ionomycin/cytochalasin-B-stimulated human neutrophils; HPLC.\nLimitations: Position of the monoglucuronide is not resolved in the abstract.\nEvidence access: Primary abstract\nDeglucuronidation of a flavonoid, luteolin monoglucuronide, during inflammation. · 2001 · https://pubmed.ncbi.nlm.nih.gov/11717168/","model_system":"Ionomycin/cytochalasin-B-stimulated human neutrophils; HPLC.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"26337a08-5cee-5cee-900e-e6d3e26ac6e4","stable_key":"import-22527305-af1f-536a-b419-7e73f08cc3ec","title":"Luteolin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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