{"id":"df2a51f7-8483-529b-a910-da09b35b832a","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:shmt1-transgene-dna-uracil","predicate":"increases","statement":"Control-diet Shmt1-transgenic mice showed approximately twice the hepatic nuclear-DNA uracil of wild-type mice.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"e75bfc01-62e9-58fe-9da5-3dedda5644f7","mechanism_event_label":"Incorrect enzyme placement was accompanied by more uracil in DNA.","subject":{"id":"2f89e068-401a-576a-8721-7ce8e50eae34","slug":"mouse-shmt1-gene","display_name":"Mouse Shmt1 gene","entity_type_key":"gene"},"object":{"id":"940ae750-a4e1-506d-bb27-ee6b99b621f1","slug":"nuclear-dna-uracil","display_name":"Uracil incorporation in nuclear DNA","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e75bfc01-62e9-58fe-9da5-3dedda5644f7","stable_key":"ec174d5a-4903-5745-8646-df0e9d4265e8:shmt1-transgene-dna-uracil-event","event_type":"biochemical_relationship","label":"Incorrect enzyme placement was accompanied by more uracil in DNA.","description":"Control-diet Shmt1-transgenic mice showed approximately twice the hepatic nuclear-DNA uracil of wild-type mice.","status":"provisional","compartment":{"slug":"nucleus","display_name":"Nucleus"},"participants":[{"entity":{"id":"2f89e068-401a-576a-8721-7ce8e50eae34","slug":"mouse-shmt1-gene","display_name":"Mouse Shmt1 gene","entity_type_key":"gene"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"940ae750-a4e1-506d-bb27-ee6b99b621f1","slug":"nuclear-dna-uracil","display_name":"Uracil incorporation in nuclear DNA","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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Study references: [macfarlane-2011] Nuclear localization of de novo thymidylate biosynthesis pathway is required to prevent uracil accumulation in DNA (2011). https://pubmed.ncbi.nlm.nih.gov/22057276/ DOI: 10.1074/jbc.m111.307629","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"f4ce1a62-9582-5f7a-84f5-a23d0e1bfc68","stable_key":"import-ec174d5a-4903-5745-8646-df0e9d4265e8","title":"Folate and folic acid: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e564d43989ece1006c95cd0748e9af6fe369074599a2eebba0a99ebff864b0dd","revision_id":"76674a33-b2a1-5e41-b71b-44399038ff7c","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}