{"id":"df0d917e-31cf-5f37-b2a3-b5cb4737c1dd","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:egf-increases-trpm6-current","predicate":"stimulates","statement":"EGF increased TRPM6-associated current in transfected HEK293 cells through EGFR-dependent signaling.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"04cc029a-5332-57e4-8038-b95dc89cfb81","mechanism_event_label":"The growth factor EGF signals through its receptor to increase magnesium-channel activity.","subject":{"id":"4479ddcc-2af8-5eb5-9532-7acaaa426d18","slug":"egf","display_name":"Epidermal growth factor","entity_type_key":"protein"},"object":{"id":"d46fc667-ae66-520d-9903-06a161280c63","slug":"trpm6-channel-current","display_name":"TRPM6-associated channel current","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"04cc029a-5332-57e4-8038-b95dc89cfb81","stable_key":"0f17db03-207f-5910-ac8e-13dfc2f378ce:egf-increases-trpm6-current-event","event_type":"biochemical_relationship","label":"The growth factor EGF signals through its receptor to increase magnesium-channel activity.","description":"EGF increased TRPM6-associated current in transfected HEK293 cells through EGFR-dependent signaling.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"844ed9d4-1202-5e1f-8db2-771dce1b0b3d","slug":"egfr","display_name":"Epidermal growth factor receptor","entity_type_key":"protein"},"role":"required-receptor","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"877a2fb0-067c-51ca-afea-f63ff16affc8","slug":"trpm6","display_name":"TRPM6","entity_type_key":"protein"},"role":"regulated-channel","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"physiological-cargo","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4479ddcc-2af8-5eb5-9532-7acaaa426d18","slug":"egf","display_name":"Epidermal growth factor","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"d46fc667-ae66-520d-9903-06a161280c63","slug":"trpm6-channel-current","display_name":"TRPM6-associated channel current","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence-system","value_text":"EGF exposure and receptor-blocking patch-clamp experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"EGF exposure and receptor-blocking patch-clamp experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"10 nM EGF for 30 minutes in the main stimulation experiment; a concentration-response series and EGFR blockade were also tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Channel-current regulation was directly tested in expression cells, not native human DCT recordings.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Magnesium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"magnesium","display_name":"Magnesium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human proteins and human-derived cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The growth factor EGF signals through its receptor to increase magnesium-channel activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[groenestege-2007-egf] Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia (2007). https://www.jci.org/articles/view/31680 DOI: 10.1172/JCI31680","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HEK293 cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"931fa9c4-1b89-5699-be17-f8d1409b3a1a","evidence_kind":"source_excerpt","locator":"Lines 1016-1028","start_line":1016,"end_line":1028,"excerpt":"### egf-increases-trpm6-current\nEGF increased TRPM6-associated current in transfected HEK293 cells through EGFR-dependent signaling.\nCondition category: normal\nnutrient_topic: Magnesium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The growth factor EGF signals through its receptor to increase magnesium-channel activity.\norganism: Human proteins and human-derived cells\ntissue_or_cell_type: HEK293 cells\nexperimental_model: EGF exposure and receptor-blocking patch-clamp experiments\nlimitations: Channel-current regulation was directly tested in expression cells, not native human DCT recordings.\nexposure: 10 nM EGF for 30 minutes in the main stimulation experiment; a concentration-response series and EGFR blockade were also tested.\nevidence-system: EGF exposure and receptor-blocking patch-clamp experiments\ntissue: HEK293 cells\n[groenestege-2007-egf] Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia (2007). https://www.jci.org/articles/view/31680 DOI: 10.1172/JCI31680","model_system":"EGF exposure and receptor-blocking patch-clamp experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [groenestege-2007-egf] Impaired basolateral sorting of pro-EGF causes isolated recessive renal hypomagnesemia (2007). https://www.jci.org/articles/view/31680 DOI: 10.1172/JCI31680","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dd101e28-1a2e-5a48-9d1e-809c77514866","stable_key":"import-0f17db03-207f-5910-ac8e-13dfc2f378ce","title":"Magnesium: cross-nutrient mechanisms and deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"e111c412f57143a17e8e65e74e8f7888b5bb9a61099873f4767f527fac19bb07","revision_id":"6b7f04f2-66ed-5859-955f-c2b50d4bf041","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}