{"id":"deda8e9c-21a5-59fc-a768-330a1d8653e6","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-trp14-electron-supply","predicate":"supports_reductive_activity","statement":"The reconstituted TRP14 system used thioredoxin reductase 1 and NADPH to drive disulfide-reduction reactions.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f3ec026b-6bd6-55dc-9ad1-4754f3a4dcf0","mechanism_event_label":"The sulfur-supply step depends on an electron supply and another enzyme.","subject":{"id":"64fac263-c4d5-51c8-beba-d061868b0ce2","slug":"txnrd1","display_name":"TXNRD1","entity_type_key":"protein"},"object":{"id":"8e8b774c-00bd-5f89-b450-e0e5c4a7fb77","slug":"txndc17","display_name":"Human thioredoxin-related protein 14 / TXNDC17","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f3ec026b-6bd6-55dc-9ad1-4754f3a4dcf0","stable_key":"a8baf7e9-80e4-5d8c-adec-9a63e84d2f21:l-cysteine-trp14-electron-supply-event","event_type":"observed_relationship","label":"The sulfur-supply step depends on an electron supply and another enzyme.","description":"The reconstituted TRP14 system used thioredoxin reductase 1 and NADPH to drive disulfide-reduction reactions.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"64fac263-c4d5-51c8-beba-d061868b0ce2","slug":"txnrd1","display_name":"TXNRD1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e8b774c-00bd-5f89-b450-e0e5c4a7fb77","slug":"txndc17","display_name":"Human thioredoxin-related protein 14 / TXNDC17","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ca899f13-50ad-55e5-ab99-329ae0038c74","slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"4aba2a5e-8d06-5304-bb01-c0402b225a94","slug":"nadph","display_name":"NADPH","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified enzyme systems, including human peroxiredoxin-2 decysteinylation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This establishes a biochemical dependency; selenium or niacin depletion/repletion was not tested in this study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Cysteine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-cysteine","display_name":"L-Cysteine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The sulfur-supply step depends on an electron supply and another enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"TRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38811853/ · DOI 10.1038/s44318-024-00117-1","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"8d264491-57f9-53eb-8b11-d2328320b3ba","evidence_kind":"source_excerpt","locator":"Lines 68-74","start_line":68,"end_line":74,"excerpt":"## l-cysteine-trp14-electron-supply\nThe sulfur-supply step depends on an electron supply and another enzyme.\nThe reconstituted TRP14 system used thioredoxin reductase 1 and NADPH to drive disulfide-reduction reactions.\nModel: Purified enzyme systems, including human peroxiredoxin-2 decysteinylation.\nLimitations: This establishes a biochemical dependency; selenium or niacin depletion/repletion was not tested in this study.\nEvidence access: Primary full text\nTRP14 is the rate-limiting enzyme for intracellular cystine reduction and regulates proteome cysteinylation. · 2024 · https://pubmed.ncbi.nlm.nih.gov/38811853/ · DOI 10.1038/s44318-024-00117-1","model_system":"Purified enzyme systems, including human peroxiredoxin-2 decysteinylation.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"654560f6-8d7f-596b-8722-48d94053cfe3","stable_key":"import-a8baf7e9-80e4-5d8c-adec-9a63e84d2f21","title":"L-Cysteine: sulfur allocation, redox supply and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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