{"id":"decc8833-1c9c-5875-a672-ed7ac5e9814f","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-retina-blue-light","predicate":"reduces","statement":"Astaxanthin pretreatment reduced ROS, mitochondrial injury and apoptosis after blue-light exposure in mouse 661W cells, with PI3K/Akt-Nrf2 pathway responses.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"2d156b83-266c-55ab-b16a-0d1efb6dd482","mechanism_event_label":"The tested cells tolerated a light challenge better after pretreatment.","subject":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"object":{"id":"203d3327-4628-5ad1-96d9-cb737fdd58a6","slug":"mouse-astaxanthin-retinal-cell-injury","display_name":"Injury in challenged mouse 661W photoreceptor-derived cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"2d156b83-266c-55ab-b16a-0d1efb6dd482","stable_key":"a8bedee0-a740-5bbb-921e-bfd144c7b68c:astaxanthin-retina-blue-light-event","event_type":"observed_relationship","label":"The tested cells tolerated a light challenge better after pretreatment.","description":"Astaxanthin pretreatment reduced ROS, mitochondrial injury and apoptosis after blue-light exposure in mouse 661W cells, with PI3K/Akt-Nrf2 pathway responses.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"1bf8b9c0-9c38-5c8a-b5da-f00b0eeca36f","slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"203d3327-4628-5ad1-96d9-cb737fdd58a6","slug":"mouse-astaxanthin-retinal-cell-injury","display_name":"Injury in challenged mouse 661W photoreceptor-derived cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"95319607-3946-51b3-8235-400ec3223692","slug":"mouse-nfe2l2","display_name":"Mouse Nrf2 / Nfe2l2","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Mouse 661W culture experiment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not evidence of proven human macular accumulation or clinical protection from screens.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Astaxanthin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"astaxanthin","display_name":"Astaxanthin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The tested cells tolerated a light challenge better after pretreatment.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Protective Effect of Astaxanthin on Blue Light Light-Emitting Diode-Induced Retinal Cell Damage via Free Radical Scavenging and Activation of PI3K/Akt/Nrf2 Pathway in 661W Cell Model. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32722441/ · DOI 10.3390/md18080387","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"46d70718-a819-5aaf-82a5-d4df3a72fe15","evidence_kind":"source_excerpt","locator":"Lines 366-372","start_line":366,"end_line":372,"excerpt":"## astaxanthin-retina-blue-light\nThe tested cells tolerated a light challenge better after pretreatment.\nAstaxanthin pretreatment reduced ROS, mitochondrial injury and apoptosis after blue-light exposure in mouse 661W cells, with PI3K/Akt-Nrf2 pathway responses.\nModel: Mouse 661W culture experiment.\nLimitations: Not evidence of proven human macular accumulation or clinical protection from screens.\nEvidence access: Primary abstract\nProtective Effect of Astaxanthin on Blue Light Light-Emitting Diode-Induced Retinal Cell Damage via Free Radical Scavenging and Activation of PI3K/Akt/Nrf2 Pathway in 661W Cell Model. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32722441/ · DOI 10.3390/md18080387","model_system":"Mouse 661W culture experiment.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4fd91ea6-78b4-5bfe-93e3-686fbef9f678","stable_key":"import-a8bedee0-a740-5bbb-921e-bfd144c7b68c","title":"Astaxanthin: transport, membrane chemistry, signaling and nutrient interactions (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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