{"id":"debf789c-aae6-5bb7-8021-d189ee10b8f6","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars2-charging","predicate":"charges","statement":"MARS2 is the mitochondrial methionyl-tRNA synthetase, connecting methionine to organelle translation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"793a01f5-46bc-5cee-84dd-0158a32cbb08","mechanism_event_label":"Mitochondria need their own charging enzyme.","subject":{"id":"72e741a1-452a-53a7-b8ec-ac441f15ad7d","slug":"mars2","display_name":"Human mitochondrial methionyl-tRNA synthetase / MARS2","entity_type_key":"protein"},"object":{"id":"2a8e2612-d381-52ab-9cd6-81247da34019","slug":"mitochondrial-methionyl-trna","display_name":"Mitochondrial methionyl-tRNA","entity_type_key":"rna"},"evidence_count":1,"mechanism_event":{"id":"793a01f5-46bc-5cee-84dd-0158a32cbb08","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars2-charging-event","event_type":"observed_relationship","label":"Mitochondria need their own charging enzyme.","description":"MARS2 is the mitochondrial methionyl-tRNA synthetase, connecting methionine to organelle translation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"72e741a1-452a-53a7-b8ec-ac441f15ad7d","slug":"mars2","display_name":"Human mitochondrial methionyl-tRNA synthetase / MARS2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"2a8e2612-d381-52ab-9cd6-81247da34019","slug":"mitochondrial-methionyl-trna","display_name":"Mitochondrial methionyl-tRNA","entity_type_key":"rna"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Established enzyme role described in the human MARS2 disease study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"This role statement is distinct from the patient-cell respiratory measurements.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Mitochondria need their own charging enzyme.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Novel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25754315/ · DOI 10.1002/humu.22781","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"13029099-5732-57a3-a67a-4a93a9f5abf6","evidence_kind":"source_excerpt","locator":"Lines 92-98","start_line":92,"end_line":98,"excerpt":"## methionine-mars2-charging\nMitochondria need their own charging enzyme.\nMARS2 is the mitochondrial methionyl-tRNA synthetase, connecting methionine to organelle translation.\nModel: Established enzyme role described in the human MARS2 disease study.\nLimitations: This role statement is distinct from the patient-cell respiratory measurements.\nEvidence access: Primary abstract\nNovel, compound heterozygous, single-nucleotide variants in MARS2 associated with developmental delay, poor growth, and sensorineural hearing loss. · 2015 · https://pubmed.ncbi.nlm.nih.gov/25754315/ · DOI 10.1002/humu.22781","model_system":"Established enzyme role described in the human MARS2 disease study.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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