{"id":"de96a4a1-2234-5279-8151-61fbf7e72457","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-distribution","predicate":"has_measured","statement":"The rat study estimated intravenous distribution volume near 51.65 L/kg ; lung and spleen tissue/plasma ratios were about 60 at 1 hour and about 240 at 2 hours, while liver, stomach and small intestine ratios were only 4–14 at 1 hour.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"e5c18c8f-5c11-54ba-88d0-cf9048b08bc9","mechanism_event_label":"Measured tissue distribution differed substantially from plasma.","subject":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"object":{"id":"dd59d5b4-da84-5e55-a7b4-6d830929cf8d","slug":"rat-cub-tissue-distribution","display_name":"Rat cucurbitacin B tissue distribution","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"e5c18c8f-5c11-54ba-88d0-cf9048b08bc9","stable_key":"2921131d-0ba0-51d3-8f59-8c4be3b9ac08:cucurbitacin-b-rat-distribution-event","event_type":"observed_relationship","label":"Measured tissue distribution differed substantially from plasma.","description":"The rat study estimated intravenous distribution volume near 51.65 L/kg ; lung and spleen tissue/plasma ratios were about 60 at 1 hour and about 240 at 2 hours, while liver, stomach and small intestine ratios were only 4–14 at 1 hour.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"a544a561-7647-55f6-8ba1-c7ab0133f968","slug":"cucurbitacin-b","display_name":"Cucurbitacin B","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"dd59d5b4-da84-5e55-a7b4-6d830929cf8d","slug":"rat-cub-tissue-distribution","display_name":"Rat cucurbitacin B tissue distribution","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"evidence_access","value_text":"Primary full text PMC6609384","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Male Wistar rat intravenous pharmacokinetics and tissue sampling; brain and heart amounts were below quantification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Total tissue ratios do not establish free intracellular concentration or a human therapeutic window.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Cucurbitacins collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"cucurbitacin","display_name":"Cucurbitacins","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"Measured tissue distribution differed substantially from plasma.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Pharmacokinetics of cucurbitacin B from Trichosanthes cucumerina L. in rats. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31272429/ · DOI 10.1186/s12906-019-2568-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"biomarker_context","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"f80baf2b-7f9c-5efc-b016-58928aadb29c","evidence_kind":"source_excerpt","locator":"Lines 356-362","start_line":356,"end_line":362,"excerpt":"## cucurbitacin-b-rat-distribution\nMeasured tissue distribution differed substantially from plasma.\nThe rat study estimated intravenous distribution volume near 51.65 L/kg ; lung and spleen tissue/plasma ratios were about 60 at 1 hour and about 240 at 2 hours, while liver, stomach and small intestine ratios were only 4–14 at 1 hour.\nModel: Male Wistar rat intravenous pharmacokinetics and tissue sampling; brain and heart amounts were below quantification.\nLimitations: Total tissue ratios do not establish free intracellular concentration or a human therapeutic window.\nEvidence access: Primary full text PMC6609384\nPharmacokinetics of cucurbitacin B from Trichosanthes cucumerina L. in rats. · 2019 · https://pubmed.ncbi.nlm.nih.gov/31272429/ · DOI 10.1186/s12906-019-2568-7","model_system":"Male Wistar rat intravenous pharmacokinetics and tissue sampling; brain and heart amounts were below quantification.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"77324310-00ce-5d28-9fc7-595fddae86b9","stable_key":"import-2921131d-0ba0-51d3-8f59-8c4be3b9ac08","title":"Cucurbitacins: thiol chemistry, cytoskeleton, metabolic dependencies and signaling (2026-09-20)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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