{"id":"de688ced-c281-5732-8be3-d2b06f740369","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-recycling-gs-block","predicate":"inhibits_supply_through","statement":"Blocking glutamine synthetase with methionine sulfoximine worsened activity-dependent synaptic depression in rat hippocampal slices.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d4af6711-b1b5-5364-b397-b5740a89240c","mechanism_event_label":"Disabling the recycling enzyme made the local supply problem worse.","subject":{"id":"bfeb5cc5-5dd2-5c26-89a5-c2f37d1a3f02","slug":"methionine-sulfoximine","display_name":"Methionine sulfoximine","entity_type_key":"small_molecule"},"object":{"id":"8f096ae7-8f24-54eb-be8e-bacdaa11d8e2","slug":"rat-glul","display_name":"Rat glutamine synthetase / Glul","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d4af6711-b1b5-5364-b397-b5740a89240c","stable_key":"b7014def-74ec-599e-8347-167e24fec1f0:glutamate-recycling-gs-block-event","event_type":"observed_relationship","label":"Disabling the recycling enzyme made the local supply problem worse.","description":"Blocking glutamine synthetase with methionine sulfoximine worsened activity-dependent synaptic depression in rat hippocampal slices.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bfeb5cc5-5dd2-5c26-89a5-c2f37d1a3f02","slug":"methionine-sulfoximine","display_name":"Methionine sulfoximine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8f096ae7-8f24-54eb-be8e-bacdaa11d8e2","slug":"rat-glul","display_name":"Rat glutamine synthetase / Glul","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"7da684a4-2641-5bc6-93ae-8c6aa384e487","slug":"glutamate","display_name":"L-Glutamate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"fa99c9c3-65e6-518d-bae9-d8459c4a8843","slug":"rat-hippocampal-local-glutamate-release","display_name":"Rat hippocampal local glutamate supply for release","entity_type_key":"cellular_process"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"8637bb39-2c30-5168-baaf-3e613d831db0","slug":"glutamine","display_name":"L-Glutamine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; 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