{"id":"de65cd3a-69d8-53ea-86c7-9ac4183f0d3a","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-sod2-yeast-temperature","predicate":"increases_with","statement":"Increasing yeast growth temperature increased both manganese content and activity of mitochondrially expressed human SOD2.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"492938d8-107e-5357-ba4e-105f32bbb99b","mechanism_event_label":"SOD2 metal loading depended on the experimental expression conditions.","subject":{"id":"eda566c2-d338-54b2-a872-0dd39daffcdf","slug":"sod2","display_name":"Human mitochondrial manganese superoxide dismutase / SOD2","entity_type_key":"protein"},"object":{"id":"68204766-fef3-5610-93c6-de6f7557c6ea","slug":"human-sod2-metallation","display_name":"Human SOD2 metallation","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"492938d8-107e-5357-ba4e-105f32bbb99b","stable_key":"be889add-cec8-500b-be89-676431432a70:mn-enz-sod2-yeast-temperature-event","event_type":"biochemical_relationship","label":"SOD2 metal loading depended on the experimental expression conditions.","description":"Increasing yeast growth temperature increased both manganese content and activity of mitochondrially expressed human SOD2.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"eda566c2-d338-54b2-a872-0dd39daffcdf","slug":"sod2","display_name":"Human mitochondrial manganese superoxide dismutase / SOD2","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"68204766-fef3-5610-93c6-de6f7557c6ea","slug":"human-sod2-metallation","display_name":"Human SOD2 metallation","entity_type_key":"cellular_process"},"role":"object","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a8082b11-c484-5792-bb81-48e8e699cbe4","slug":"manganese-ion","display_name":"Mn2+","entity_type_key":"ion"},"role":"loaded cofactor","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human SOD2 expressed in Saccharomyces cerevisiae and purified from yeast mitochondria","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Heterologous expression; metallation and reconstitution","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Human protein in yeast; this finding is not a recommendation to alter human body temperature.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Manganese research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"manganese","display_name":"Manganese","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Human protein in Saccharomyces cerevisiae","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SOD2 metal loading depended on the experimental expression conditions.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[mn-enz-22561997] Metallation state of human manganese superoxide dismutase expressed in Saccharomyces cerevisiae. (2012). https://pubmed.ncbi.nlm.nih.gov/22561997/ DOI: 10.1016/j.abb.2012.04.016","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Yeast mitochondria","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"045e7ccb-7730-5846-9861-ed9991d857e8","evidence_kind":"source_excerpt","locator":"Lines 483-493","start_line":483,"end_line":493,"excerpt":"### mn-enz-sod2-yeast-temperature\nIncreasing yeast growth temperature increased both manganese content and activity of mitochondrially expressed human SOD2.\nCondition category: normal\nnutrient_topic: Manganese research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SOD2 metal loading depended on the experimental expression conditions.\norganism: Human protein in Saccharomyces cerevisiae\ntissue_or_cell_type: Yeast mitochondria\nexperimental_model: Human SOD2 expressed in Saccharomyces cerevisiae and purified from yeast mitochondria\nlimitations: Human protein in yeast; this finding is not a recommendation to alter human body temperature.\nexposure: Heterologous expression; metallation and reconstitution\n[mn-enz-22561997] Metallation state of human manganese superoxide dismutase expressed in Saccharomyces cerevisiae. (2012). https://pubmed.ncbi.nlm.nih.gov/22561997/ DOI: 10.1016/j.abb.2012.04.016","model_system":"Human SOD2 expressed in Saccharomyces cerevisiae and purified from yeast mitochondria","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [mn-enz-22561997] Metallation state of human manganese superoxide dismutase expressed in Saccharomyces cerevisiae. (2012). https://pubmed.ncbi.nlm.nih.gov/22561997/ DOI: 10.1016/j.abb.2012.04.016","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"03224387-8a73-5b4f-906d-9f0be6625b7f","stable_key":"import-be889add-cec8-500b-be89-676431432a70","title":"Manganese: enzyme cofactors, glycosylation, transport and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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