{"id":"de5db900-aa57-58f3-83ff-862561660418","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-dnaj-atpase","predicate":"blocks_dnaj_stimulation_of","statement":"Myricetin blocked DnaJ-stimulated DnaK ATPase activity with little effect on intrinsic or GrpE-stimulated turnover.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"7097d0cd-3ae1-531e-a7cc-8fecfd319d47","mechanism_event_label":"The same enzyme responds differently to its partner.","subject":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"object":{"id":"3ef06b48-8daf-5aba-8f16-d43fb24e560a","slug":"ecoli-dnak","display_name":"Escherichia coli DnaK chaperone","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"7097d0cd-3ae1-531e-a7cc-8fecfd319d47","stable_key":"8182578e-8d21-598e-a3cb-e62d480d2251:myricetin-dnaj-atpase-event","event_type":"observed_relationship","label":"The same enzyme responds differently to its partner.","description":"Myricetin blocked DnaJ-stimulated DnaK ATPase activity with little effect on intrinsic or GrpE-stimulated turnover.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"fafe05e7-4874-56e6-a42a-2ebf1ab2dd57","slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3ef06b48-8daf-5aba-8f16-d43fb24e560a","slug":"ecoli-dnak","display_name":"Escherichia coli DnaK chaperone","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3e8c3c69-f973-51f7-bdfe-15fb3b1459d9","slug":"ecoli-dnaj","display_name":"Escherichia coli DnaJ co-chaperone","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"907f1f4c-2b61-58d7-8ec1-490c86fdd882","slug":"ecoli-grpe","display_name":"Escherichia coli GrpE nucleotide-exchange factor","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified bacterial DnaK/DnaJ/GrpE system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Not demonstrated infection treatment or host chaperone inhibition.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Myricetin collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"myricetin","display_name":"Myricetin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"The same enzyme responds differently to its partner.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Chemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21338918/ · DOI 10.1016/j.chembiol.2010.12.010","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"0a1b2dd6-0e41-57b2-8202-bc9d78501644","evidence_kind":"source_excerpt","locator":"Lines 588-594","start_line":588,"end_line":594,"excerpt":"## myricetin-dnaj-atpase\nThe same enzyme responds differently to its partner.\nMyricetin blocked DnaJ-stimulated DnaK ATPase activity with little effect on intrinsic or GrpE-stimulated turnover.\nModel: Purified bacterial DnaK/DnaJ/GrpE system.\nLimitations: Not demonstrated infection treatment or host chaperone inhibition.\nEvidence access: Primary full text\nChemical screens against a reconstituted multiprotein complex: myricetin blocks DnaJ regulation of DnaK through an allosteric mechanism. · 2011 · https://pubmed.ncbi.nlm.nih.gov/21338918/ · DOI 10.1016/j.chembiol.2010.12.010","model_system":"Purified bacterial DnaK/DnaJ/GrpE system.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"58045cd9-3b27-5469-bb4b-e1c6a9bdca34","stable_key":"import-8182578e-8d21-598e-a3cb-e62d480d2251","title":"Myricetin: metabolism, immune signaling, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"0abf512275cf3aae9e4ec8544f1cde97e0155d0cfb5be12a193cdd5a898386a7","revision_id":"422e342f-a55c-5e73-ba98-43df560f49b9","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}