{"id":"de424bc5-a3ea-5948-b494-ff2705aa46f9","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-serca-transition","predicate":"stabilizes_transition_like_state","statement":"Vanadate occupied the catalytic site of calcium-free SERCA as planar VO3 with water and magnesium in a dephosphorylation-transition-like conformation.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f6dfc0e5-3d5b-58e8-a5f1-6716614242df","mechanism_event_label":"A phosphate analogue can trap a calcium pump in a catalytic state.","subject":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"object":{"id":"8e6408b2-3067-546d-ad43-b77fc0a917fb","slug":"rabbit-atp2a1-serca1a","display_name":"Rabbit SERCA1a / ATP2A1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"f6dfc0e5-3d5b-58e8-a5f1-6716614242df","stable_key":"335be270-ea4a-5c8e-ad04-964fd22a439e:vanadium-serca-transition-event","event_type":"observed_relationship","label":"A phosphate analogue can trap a calcium pump in a catalytic state.","description":"Vanadate occupied the catalytic site of calcium-free SERCA as planar VO3 with water and magnesium in a dephosphorylation-transition-like conformation.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"bef86a6e-28a0-5df9-9306-3f05991aa159","slug":"vanadate-v","display_name":"Vanadate(V), protonation/speciation dependent","entity_type_key":"chemical_species"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"8e6408b2-3067-546d-ad43-b77fc0a917fb","slug":"rabbit-atp2a1-serca1a","display_name":"Rabbit SERCA1a / ATP2A1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c5cbad45-22c6-594b-ab6b-71880dab1b24","slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"bff427ab-35f9-59c2-bb24-fd5953bbaec2","slug":"magnesium-ion","display_name":"Mg2+","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"e359bc15-e675-5d83-b0fe-1d70814e130b","slug":"calcium-ion","display_name":"Calcium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""},{"entity":{"id":"b28dfc54-a5ef-5f9e-ac1f-890b68e58dc3","slug":"inorganic-phosphate","display_name":"Inorganic phosphate","entity_type_key":"chemical_species"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":5,"notes":""},{"entity":{"id":"58b974f1-d389-5bf6-81cd-889c44442c42","slug":"atp","display_name":"ATP","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":6,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract and RCSB PDB 5A3Q author deposition","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Rabbit SERCA structure, deposition 5A3Q; primary abstract plus author-deposited structure identity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structure used nucleotide analogues and thapsigargin; not a measured human intracellular calcium effect.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vanadium collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vanadium","display_name":"Vanadium","entity_type_key":"nutrient_element"}},{"dimension":"plain_language","value_text":"A phosphate analogue can trap a calcium pump in a catalytic state.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Crystal Structure of the Vanadate-Inhibited Ca(2+)-ATPase. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27050689/ · DOI 10.1016/j.str.2016.02.018","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"89f5a3d8-092c-5f50-a768-53420c223f42","evidence_kind":"source_excerpt","locator":"Lines 254-260","start_line":254,"end_line":260,"excerpt":"## vanadium-serca-transition\nA phosphate analogue can trap a calcium pump in a catalytic state.\nVanadate occupied the catalytic site of calcium-free SERCA as planar VO3 with water and magnesium in a dephosphorylation-transition-like conformation.\nModel: Rabbit SERCA structure, deposition 5A3Q; primary abstract plus author-deposited structure identity.\nLimitations: Structure used nucleotide analogues and thapsigargin; not a measured human intracellular calcium effect.\nEvidence access: Primary abstract and RCSB PDB 5A3Q author deposition\nCrystal Structure of the Vanadate-Inhibited Ca(2+)-ATPase. · 2016 · https://pubmed.ncbi.nlm.nih.gov/27050689/ · DOI 10.1016/j.str.2016.02.018","model_system":"Rabbit SERCA structure, deposition 5A3Q; primary abstract plus author-deposited structure identity.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e4d5d0ba-529b-540f-96ed-e719d116ccfd","stable_key":"import-335be270-ea4a-5c8e-ad04-964fd22a439e","title":"Vanadium: speciation, phosphate-sensitive enzymes and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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