{"id":"de3ff81d-307a-5106-b4bd-d604d50266a9","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-monocyte-binding","predicate":"reduces","statement":"At 1-3 mM pretreatment, ergothioneine reduced U937 monocyte attachment to IL-1beta-stimulated human aortic endothelial cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"5493b14e-97c1-5f97-bc24-9e844677ab17","mechanism_event_label":"A functional adhesion measurement followed the expression changes.","subject":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"object":{"id":"3faf5f3a-9f01-5d30-b2a9-0a64969eb43a","slug":"human-monocyte-endothelial-adhesion","display_name":"Human U937 monocyte adhesion to endothelial cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"5493b14e-97c1-5f97-bc24-9e844677ab17","stable_key":"59b18080-c560-563d-abc5-bac4ee82a27c:ergothioneine-monocyte-binding-event","event_type":"observed_relationship","label":"A functional adhesion measurement followed the expression changes.","description":"At 1-3 mM pretreatment, ergothioneine reduced U937 monocyte attachment to IL-1beta-stimulated human aortic endothelial cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"939c92c3-50ea-5a4a-9654-8e3b94448132","slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3faf5f3a-9f01-5d30-b2a9-0a64969eb43a","slug":"human-monocyte-endothelial-adhesion","display_name":"Human U937 monocyte adhesion to endothelial cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human cultured-cell binding assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"High in vitro exposure; the cells and adhesion markers belong to one study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Ergothioneine collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"ergothioneine","display_name":"L-Ergothioneine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A functional adhesion measurement followed the expression changes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"The bioactive agent ergothioneine, a key component of dietary mushrooms, inhibits monocyte binding to endothelial cells characteristic of early cardiovascular disease. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21091247/ · DOI 10.1089/jmf.2009.0194","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"327f1276-3981-5b0e-bcc4-b01fa17ac2ca","evidence_kind":"source_excerpt","locator":"Lines 344-350","start_line":344,"end_line":350,"excerpt":"## ergothioneine-monocyte-binding\nA functional adhesion measurement followed the expression changes.\nAt 1-3 mM pretreatment, ergothioneine reduced U937 monocyte attachment to IL-1beta-stimulated human aortic endothelial cells.\nModel: Human cultured-cell binding assay.\nLimitations: High in vitro exposure; the cells and adhesion markers belong to one study.\nEvidence access: Primary abstract\nThe bioactive agent ergothioneine, a key component of dietary mushrooms, inhibits monocyte binding to endothelial cells characteristic of early cardiovascular disease. · 2010 · https://pubmed.ncbi.nlm.nih.gov/21091247/ · DOI 10.1089/jmf.2009.0194","model_system":"Human cultured-cell binding assay.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"2f037d98-f3d0-5dbe-80d8-90b738f130d6","stable_key":"import-59b18080-c560-563d-abc5-bac4ee82a27c","title":"Ergothioneine: transport, redox chemistry and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"b6def8119f03cfabeec2fb55ba924340dc612e1dd42f05404201fa2b7ced5259","revision_id":"f88c38e6-ffb5-5a68-83ca-4610ca2c2df4","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}