{"id":"de1aced9-ab4b-5b64-acb9-4fcaa6b96f3d","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:atg7-loss-worsens-hypokalemic-concentrating-defect","predicate":"decreases","statement":"Principal-cell Atg7 deletion worsened urine-concentrating impairment during potassium depletion rather than rescuing it.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"50960d47-0ea3-5e45-a5ae-c13fec9d07e2","mechanism_event_label":"Blocking one protein-disposal pathway did not restore water balance; channel phosphorylation and localization still mattered.","subject":{"id":"19cb3d00-b0aa-5071-a0e5-e5550adf7409","slug":"atg7-principal-cell-deletion","display_name":"Principal-cell Atg7 deletion","entity_type_key":"gene"},"object":{"id":"27ecded3-49fb-583f-9169-da5ffca3062e","slug":"urine-concentrating-capacity","display_name":"Renal urine concentrating capacity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"50960d47-0ea3-5e45-a5ae-c13fec9d07e2","stable_key":"5102beb6-9f61-500e-af10-9ac63649e0b7:atg7-loss-worsens-hypokalemic-concentrating-defect-event","event_type":"biochemical_relationship","label":"Blocking one protein-disposal pathway did not restore water balance; 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unverified.","entity":null},{"dimension":"endpoint","value_text":"Principal-cell Atg7 deletion worsened urine-concentrating impairment during potassium depletion rather than rescuing it.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental-exposure","value_text":"Principal-cell Atg7 conditional knockout and floxed control mice on normal or potassium-free diet for two weeks.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Principal-cell Atg7 conditional knockout and floxed control mice on normal or potassium-free diet for two weeks.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Knockout already altered basal urine concentration and caused compensatory degradation changes; this is not evidence against every role for autophagy.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium","display_name":"Potassium","entity_type_key":"nutrient_element"}},{"dimension":"organism","value_text":"Mus musculus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Blocking one protein-disposal pathway did not restore water balance; channel phosphorylation and localization still mattered.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[kim-2019-atg7-aqp2] Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia (2019). https://www.nature.com/articles/s41598-019-39702-4 DOI: 10.1038/s41598-019-39702-4","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"collecting-duct principal cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"3f937f88-70c6-536e-bb2a-0e62d37bbcfb","evidence_kind":"source_excerpt","locator":"Lines 1425-1436","start_line":1425,"end_line":1436,"excerpt":"### atg7-loss-worsens-hypokalemic-concentrating-defect\nPrincipal-cell Atg7 deletion worsened urine-concentrating impairment during potassium depletion rather than rescuing it.\nCondition category: machinery_impairment\nnutrient_topic: Potassium research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Blocking one protein-disposal pathway did not restore water balance; channel phosphorylation and localization still mattered.\norganism: Mus musculus\ntissue_or_cell_type: collecting-duct principal cells\nexperimental_model: Principal-cell Atg7 conditional knockout and floxed control mice on normal or potassium-free diet for two weeks.\nlimitations: Knockout already altered basal urine concentration and caused compensatory degradation changes; this is not evidence against every role for autophagy.\nexperimental-exposure: Principal-cell Atg7 conditional knockout and floxed control mice on normal or potassium-free diet for two weeks.\nendpoint: Principal-cell Atg7 deletion worsened urine-concentrating impairment during potassium depletion rather than rescuing it.\n[kim-2019-atg7-aqp2] Atg7-dependent canonical autophagy regulates the degradation of aquaporin 2 in prolonged hypokalemia (2019). https://www.nature.com/articles/s41598-019-39702-4 DOI: 10.1038/s41598-019-39702-4","model_system":"Principal-cell Atg7 conditional knockout and floxed control mice on normal or potassium-free diet for two weeks.","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. 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