{"id":"ddf5165c-ef58-500b-aa22-0c37d894ef86","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-scarb1-uptake","predicate":"supports_uptake","statement":"Blocking SR-BI with an extracellular antibody or BLT1 reduced RRR-alpha-tocopherol uptake by human Caco-2 TC7 cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"71bc7e1c-b83c-5a0e-b9e6-93a20d5edd75","mechanism_event_label":"SR-BI helped these intestinal cells take up vitamin E.","subject":{"id":"e00491c3-4448-559c-9012-57f5ce969157","slug":"scarb1","display_name":"Scavenger receptor class B type 1 / SCARB1","entity_type_key":"protein"},"object":{"id":"3436f320-f9dd-5606-9a37-507e5944ee6c","slug":"intestinal-alpha-tocopherol-uptake","display_name":"Intestinal alpha-tocopherol uptake","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"71bc7e1c-b83c-5a0e-b9e6-93a20d5edd75","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-scarb1-uptake-event","event_type":"biochemical_relationship","label":"SR-BI helped these intestinal cells take up vitamin E.","description":"Blocking SR-BI with an extracellular antibody or BLT1 reduced RRR-alpha-tocopherol uptake by human Caco-2 TC7 cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"e00491c3-4448-559c-9012-57f5ce969157","slug":"scarb1","display_name":"Scavenger receptor class B type 1 / SCARB1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3436f320-f9dd-5606-9a37-507e5944ee6c","slug":"intestinal-alpha-tocopherol-uptake","display_name":"Intestinal alpha-tocopherol uptake","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ae6048a7-d112-5d3a-8e7c-ad5a152642f9","slug":"rrr-alpha-tocopherol","display_name":"RRR-alpha-tocopherol","entity_type_key":"small_molecule"},"role":"uptake_substrate","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Differentiated Caco-2 TC7 monolayers","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"60-min pretreatment: antibody 3.75 µg/mL or BLT1 10 µM; 40 µM micellar RRR-alpha-tocopherol for 60 min.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Inhibitor/antibody evidence; contribution under these conditions is not the sole absorption route.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"SR-BI helped these intestinal cells take up vitamin E.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[reboul2006] Scavenger receptor class B type I (SR-BI) is involved in vitamin E transport across the enterocyte. (2006). https://pubmed.ncbi.nlm.nih.gov/16380385/ DOI: 10.1074/jbc.m509042200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Intestinal epithelial cell model","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"01122e90-259c-5509-8fa2-034f96c878fa","evidence_kind":"source_excerpt","locator":"Lines 181-192","start_line":181,"end_line":192,"excerpt":"### ve-transport-scarb1-uptake\nBlocking SR-BI with an extracellular antibody or BLT1 reduced RRR-alpha-tocopherol uptake by human Caco-2 TC7 cells.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: SR-BI helped these intestinal cells take up vitamin E.\norganism: Homo sapiens\ntissue_or_cell_type: Intestinal epithelial cell model\nexperimental_model: Differentiated Caco-2 TC7 monolayers\nlimitations: Inhibitor/antibody evidence; contribution under these conditions is not the sole absorption route.\nexposure: 60-min pretreatment: antibody 3.75 µg/mL or BLT1 10 µM; 40 µM micellar RRR-alpha-tocopherol for 60 min.\ncross_nutrient: false\n[reboul2006] Scavenger receptor class B type I (SR-BI) is involved in vitamin E transport across the enterocyte. 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