{"id":"ddf10c1b-1f15-5dd6-a42d-7fb9fdad6b73","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars-phosphorylation","predicate":"phosphorylates","statement":"Oxidative-stress experiments identified ERK-dependent MARS1 phosphorylation at Ser209 and Ser825.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ecc3ee6e-64e5-5048-a1cc-89acbbd0ad2b","mechanism_event_label":"Stress signaling changes how the charging enzyme behaves.","subject":{"id":"cc602857-e4e2-5fb7-9a9d-62a2e810fa48","slug":"human-erk1-erk2","display_name":"Human ERK1/ERK2 kinases","entity_type_key":"protein_set"},"object":{"id":"d66988d4-32f5-5d3f-9d5e-e3035886d544","slug":"mars1","display_name":"Human cytosolic methionyl-tRNA synthetase / MARS1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ecc3ee6e-64e5-5048-a1cc-89acbbd0ad2b","stable_key":"41a89233-da9a-5b7b-9c18-e19bbadcfe2d:methionine-mars-phosphorylation-event","event_type":"observed_relationship","label":"Stress signaling changes how the charging enzyme behaves.","description":"Oxidative-stress experiments identified ERK-dependent MARS1 phosphorylation at Ser209 and Ser825.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cc602857-e4e2-5fb7-9a9d-62a2e810fa48","slug":"human-erk1-erk2","display_name":"Human ERK1/ERK2 kinases","entity_type_key":"protein_set"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d66988d4-32f5-5d3f-9d5e-e3035886d544","slug":"mars1","display_name":"Human cytosolic methionyl-tRNA synthetase / MARS1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d39f561-740b-5f67-bba7-8a72ef612a99","slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human HeLa/HEK293T cells, kinase assays and site-directed mutants.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Arsenite/peroxide and engineered constructs do not represent routine dietary exposure.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Methionine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"methionine","display_name":"L-Methionine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Stress signaling changes how the charging enzyme behaves.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Promiscuous methionyl-tRNA synthetase mediates adaptive mistranslation to protect cells against oxidative stress. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25097229/ · DOI 10.1242/jcs.152470","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f89c3c0d-9b5e-58c8-95fa-d6cceb91c3df","evidence_kind":"source_excerpt","locator":"Lines 68-74","start_line":68,"end_line":74,"excerpt":"## methionine-mars-phosphorylation\nStress signaling changes how the charging enzyme behaves.\nOxidative-stress experiments identified ERK-dependent MARS1 phosphorylation at Ser209 and Ser825.\nModel: Human HeLa/HEK293T cells, kinase assays and site-directed mutants.\nLimitations: Arsenite/peroxide and engineered constructs do not represent routine dietary exposure.\nEvidence access: Primary full text\nPromiscuous methionyl-tRNA synthetase mediates adaptive mistranslation to protect cells against oxidative stress. · 2014 · https://pubmed.ncbi.nlm.nih.gov/25097229/ · DOI 10.1242/jcs.152470","model_system":"Human HeLa/HEK293T cells, kinase assays and site-directed mutants.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d969b004-ccc2-5bb4-9109-8ac649137ad1","stable_key":"import-41a89233-da9a-5b7b-9c18-e19bbadcfe2d","title":"L-Methionine: transport, methylation, sulfur metabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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