{"id":"ddeda9ce-2ded-54b7-a707-668232e85cfb","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-abca1-apoa1-efflux","predicate":"supports_efflux","statement":"Expression of human ABCA1 in BHK cells increased alpha-tocopherol efflux to apoA-I.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d0858856-0c0a-5fdf-ab04-d9185b407156","mechanism_event_label":"ABCA1 helped transfer cellular vitamin E to an extracellular lipoprotein protein.","subject":{"id":"c5efa7cf-7e87-5809-8159-8d3f17013501","slug":"abca1","display_name":"Human ATP-binding cassette transporter A1","entity_type_key":"protein"},"object":{"id":"3b19df9f-fc4e-5d7f-ab27-f46ad8101269","slug":"apoa1-directed-alpha-tocopherol-efflux","display_name":"ApoA-I-directed alpha-tocopherol efflux","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d0858856-0c0a-5fdf-ab04-d9185b407156","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ve-transport-abca1-apoa1-efflux-event","event_type":"biochemical_relationship","label":"ABCA1 helped transfer cellular vitamin E to an extracellular lipoprotein protein.","description":"Expression of human ABCA1 in BHK cells increased alpha-tocopherol efflux to apoA-I.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"c5efa7cf-7e87-5809-8159-8d3f17013501","slug":"abca1","display_name":"Human ATP-binding cassette transporter A1","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"3b19df9f-fc4e-5d7f-ab27-f46ad8101269","slug":"apoa1-directed-alpha-tocopherol-efflux","display_name":"ApoA-I-directed alpha-tocopherol efflux","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"cargo","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"dc58a736-a6f1-52cd-8973-65b08d49efc7","slug":"apoa1","display_name":"Human apolipoprotein A-I","entity_type_key":"protein"},"role":"extracellular_acceptor","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human ABCA1 gain of function in BHK cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Radiolabeled alpha-tocopherol; 2-hour efflux to 5 µg/mL apoA-I with 1 mg/mL BSA.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Engineered cell assay; does not identify ABCA1 as the only vitamin E exporter.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Human protein in hamster cells","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"ABCA1 helped transfer cellular vitamin E to an extracellular lipoprotein protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[oram2001] ATP-binding cassette transporter A1 mediates cellular secretion of alpha-tocopherol. (2001). https://pubmed.ncbi.nlm.nih.gov/11546785/ DOI: 10.1074/jbc.m106984200","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"BHK kidney-derived cell line","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"69191f84-4cfb-5245-8815-8f3f795413a7","evidence_kind":"source_excerpt","locator":"Lines 272-283","start_line":272,"end_line":283,"excerpt":"### ve-transport-abca1-apoa1-efflux\nExpression of human ABCA1 in BHK cells increased alpha-tocopherol efflux to apoA-I.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: ABCA1 helped transfer cellular vitamin E to an extracellular lipoprotein protein.\norganism: Human protein in hamster cells\ntissue_or_cell_type: BHK kidney-derived cell line\nexperimental_model: Human ABCA1 gain of function in BHK cells\nlimitations: Engineered cell assay; does not identify ABCA1 as the only vitamin E exporter.\nexposure: Radiolabeled alpha-tocopherol; 2-hour efflux to 5 µg/mL apoA-I with 1 mg/mL BSA.\ncross_nutrient: false\n[oram2001] ATP-binding cassette transporter A1 mediates cellular secretion of alpha-tocopherol. (2001). https://pubmed.ncbi.nlm.nih.gov/11546785/ DOI: 10.1074/jbc.m106984200","model_system":"Human ABCA1 gain of function in BHK cells","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [oram2001] ATP-binding cassette transporter A1 mediates cellular secretion of alpha-tocopherol. (2001). https://pubmed.ncbi.nlm.nih.gov/11546785/ DOI: 10.1074/jbc.m106984200","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. 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