{"id":"dd5704e6-c1ef-57c5-9df8-9052061b3f32","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldc-host-effect","predicate":"disruption_changes","statement":"Gnotobiotic mice colonized with the fldC-mutant strain had reduced gut and circulating indolepropionate and increased intestinal permeability compared with wild-type colonization.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"20048133-a384-5d4d-9aac-35905296fd00","mechanism_event_label":"A microbial genetic change altered a host barrier phenotype.","subject":{"id":"b3e7b70f-69ce-591b-96fa-389bae9a7ed9","slug":"c-sporogenes-fldc","display_name":"Clostridium sporogenes FldC","entity_type_key":"protein"},"object":{"id":"4509b8f7-cad2-57c3-84b4-75256713c083","slug":"mouse-trp-gut-permeability","display_name":"Mouse intestinal permeability in microbial tryptophan experiments","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"20048133-a384-5d4d-9aac-35905296fd00","stable_key":"584c58f5-ab9f-53f3-97a9-55783db943b0:tryptophan-fldc-host-effect-event","event_type":"observed_relationship","label":"A microbial genetic change altered a host barrier phenotype.","description":"Gnotobiotic mice colonized with the fldC-mutant strain had reduced gut and circulating indolepropionate and increased intestinal permeability compared with wild-type colonization.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b3e7b70f-69ce-591b-96fa-389bae9a7ed9","slug":"c-sporogenes-fldc","display_name":"Clostridium sporogenes FldC","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4509b8f7-cad2-57c3-84b4-75256713c083","slug":"mouse-trp-gut-permeability","display_name":"Mouse intestinal permeability in microbial tryptophan experiments","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"769339cb-213b-559e-acc0-07ed00368b94","slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"813fc8bf-f15a-5201-91e3-d1579084376d","slug":"indole-3-propionic-acid","display_name":"Indole-3-propionic acid / indolepropionate","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text, host colonization results","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Gnotobiotic mouse colonization.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Several metabolites changed; this comparison alone does not prove indolepropionate is the sole mediator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Tryptophan collection; molecular form, preparation, species, exposure and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"l-tryptophan","display_name":"L-Tryptophan","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A microbial genetic change altered a host barrier phenotype.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"A gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"34d1d89e-1bae-53ed-877a-0561d836f332","evidence_kind":"source_excerpt","locator":"Lines 514-520","start_line":514,"end_line":520,"excerpt":"## tryptophan-fldc-host-effect\nA microbial genetic change altered a host barrier phenotype.\nGnotobiotic mice colonized with the fldC-mutant strain had reduced gut and circulating indolepropionate and increased intestinal permeability compared with wild-type colonization.\nModel: Gnotobiotic mouse colonization.\nLimitations: Several metabolites changed; this comparison alone does not prove indolepropionate is the sole mediator.\nEvidence access: Primary full text, host colonization results\nA gut bacterial pathway metabolizes aromatic amino acids into nine circulating metabolites. · 2017 · https://pubmed.ncbi.nlm.nih.gov/29168502/ · DOI 10.1038/nature24661","model_system":"Gnotobiotic mouse colonization.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"73f9d3e7-fdc9-5418-8f3c-f4ef145f6efa","stable_key":"import-584c58f5-ab9f-53f3-97a9-55783db943b0","title":"Tryptophan: transport, protein synthesis, neuroactive metabolites, NAD and microbial pathways (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-abstract references and experimental limitations individually identified. 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