{"id":"dd4ca2c2-f597-5ee8-95ac-498038d4f254","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-prostate-srebp2","predicate":"stabilizes_via_propionylation","statement":"Propionyl-CoA-dependent site-specific lysine propionylation stabilized nuclear SREBP2 and increased its transcriptional activity in the reported prostate-cancer models.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"ad7e70e1-18c1-5bb7-8a5d-4af825cd4f02","mechanism_event_label":"A breakdown product changed the stability of a lipid-regulating transcription factor.","subject":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"object":{"id":"d6d06d2b-a5c5-5254-98b2-8f9051537109","slug":"prostate-model-srebp2","display_name":"SREBP2 in the prostate-cancer study, species unresolved here","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"ad7e70e1-18c1-5bb7-8a5d-4af825cd4f02","stable_key":"09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2:isoleucine-prostate-srebp2-event","event_type":"observed_relationship","label":"A breakdown product changed the stability of a lipid-regulating transcription factor.","description":"Propionyl-CoA-dependent site-specific lysine propionylation stabilized nuclear SREBP2 and increased its transcriptional activity in the reported prostate-cancer models.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"69ac0570-94d8-59d8-85ef-7f67cf302d16","slug":"propionyl-coa","display_name":"Propionyl-CoA","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"d6d06d2b-a5c5-5254-98b2-8f9051537109","slug":"prostate-model-srebp2","display_name":"SREBP2 in the prostate-cancer study, species unresolved here","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c85b327e-f132-5d9a-8e4b-c2976fcc0373","slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"ae40617e-3df7-5d48-8dd2-f16a0d3a8a25","slug":"valine","display_name":"L-Valine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Primary prostate-cancer mechanistic study; exact construct and lysine site not resolved in accessed abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Site identity is not guessed; clinical target engagement was not established.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Isoleucine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"isoleucine","display_name":"L-Isoleucine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"A breakdown product changed the stability of a lipid-regulating transcription factor.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Isoleucine and valine promote prostate cancer progression via propionyl-CoA-mediated cholesterol metabolism. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42625032/ · DOI 10.1038/s42255-026-01583-z","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5f37db37-617f-5856-a22b-a6e3e7854948","evidence_kind":"source_excerpt","locator":"Lines 506-512","start_line":506,"end_line":512,"excerpt":"## isoleucine-prostate-srebp2\nA breakdown product changed the stability of a lipid-regulating transcription factor.\nPropionyl-CoA-dependent site-specific lysine propionylation stabilized nuclear SREBP2 and increased its transcriptional activity in the reported prostate-cancer models.\nModel: Primary prostate-cancer mechanistic study; exact construct and lysine site not resolved in accessed abstract.\nLimitations: Site identity is not guessed; clinical target engagement was not established.\nEvidence access: Primary abstract\nIsoleucine and valine promote prostate cancer progression via propionyl-CoA-mediated cholesterol metabolism. · 2026 · https://pubmed.ncbi.nlm.nih.gov/42625032/ · DOI 10.1038/s42255-026-01583-z","model_system":"Primary prostate-cancer mechanistic study; exact construct and lysine site not resolved in accessed abstract.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"4b00f118-00fd-5cef-be97-13125eb958e9","stable_key":"import-09b23d0d-35e2-51ff-b1aa-b4f9227e4fa2","title":"L-Isoleucine: transport, translation, catabolism and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; 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