{"id":"dd4b97f0-488d-55bf-b8fb-63e17c3b23e5","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-lung-apoptosis","predicate":"increases_tested","statement":"SAC at 20 mM for 72 hours increased apoptosis in the tested lung cancer cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"cdd4e37c-2321-545d-a943-f8c9c045f473","mechanism_event_label":"Cell death was measured alongside signaling changes.","subject":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"object":{"id":"6581b097-218c-5d35-9d71-7d91d02ff0f2","slug":"human-sac-lung-apoptosis","display_name":"Apoptosis in SAC-treated HCC827/NCI-H1975 cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"cdd4e37c-2321-545d-a943-f8c9c045f473","stable_key":"d96b230c-481b-535b-930c-04eb87ec7f02:s-allylcysteine-lung-apoptosis-event","event_type":"observed_relationship","label":"Cell death was measured alongside signaling changes.","description":"SAC at 20 mM for 72 hours increased apoptosis in the tested lung cancer cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8c2c295d-68b3-5e62-b1a7-00fcf915e3cc","slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6581b097-218c-5d35-9d71-7d91d02ff0f2","slug":"human-sac-lung-apoptosis","display_name":"Apoptosis in SAC-treated HCC827/NCI-H1975 cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"acting_entity","value_text":"s-allylcysteine","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"dose","value_text":"10 or 20 mM SAC; 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species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"s-allylcysteine","display_name":"S-allyl-L-cysteine / SAC","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Cell death was measured alongside signaling changes.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[33136700] S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33136700/ · DOI 10.1097/CAD.0000000000001015","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"Cell culture","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"HCC827 and NCI-H1975 lung cancer cells","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"15f65c84-69fa-570b-9551-c02a1d87157e","evidence_kind":"source_excerpt","locator":"Lines 329-336","start_line":329,"end_line":336,"excerpt":"## s-allylcysteine-lung-apoptosis\nCell death was measured alongside signaling changes.\nSAC at 20 mM for 72 hours increased apoptosis in the tested lung cancer cells.\nModel: HCC827 and NCI-H1975 lung cancer cells\nLimitations: A cell-death endpoint is not a demonstrated cancer treatment in humans.\nEvidence access: Primary abstract\n[33136700] S-allylcysteine induces cytotoxic effects in two human lung cancer cell lines via induction of oxidative damage, downregulation of Nrf2 and NF-κB, and apoptosis. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33136700/ · DOI 10.1097/CAD.0000000000001015\nStructured context: {\"organism\": \"Homo sapiens\", \"tissue_or_cell_type\": \"HCC827 and NCI-H1975 lung cancer cells\", \"dose\": \"10 or 20 mM SAC; 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