{"id":"dd2893ba-967e-57e5-b17a-e295f2ee615f","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-c3-convertase-null","predicate":"no_detected_change_in_recorded_comparison","statement":"RA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"00205326-50d2-5613-bfce-ab8b6f42af32","mechanism_event_label":"RA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.","subject":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"object":{"id":"90386e04-048b-5a08-9828-a12d82432451","slug":"human-c3-convertase","display_name":"Human complement C3 convertase, pathway specified by assay","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"00205326-50d2-5613-bfce-ab8b6f42af32","stable_key":"ca8e9e6a-6953-51ec-b712-2b06398a416d:ra-c3-convertase-null-event","event_type":"experimental_observation","label":"RA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.","description":"### ra-c3-convertase-null\n\nRA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.\nThis rules against calling the attachment result C3-convertase inhibition.\nModel and exposure: Purified complement system.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6072a4ae-d563-5466-a1b2-6d280255d346","slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"RA up to 10 mM","sequence_order":0,"notes":""},{"entity":{"id":"90386e04-048b-5a08-9828-a12d82432451","slug":"human-c3-convertase","display_name":"Human complement C3 convertase, pathway specified by assay","entity_type_key":"protein_complex"},"role":"measured outcome","stoichiometry":null,"state_label":"no_detected_change","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"RA up to 10 mM\", \"comparator\": \"No RA\", \"endpoint\": \"RA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.\", \"effect_direction\": \"no_detected_change\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Purified complement system.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Rosmarinic acid collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"rosmarinic-acid","display_name":"Rosmarinic acid","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"RA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference ra-c3-convertase-null; This rules against calling the attachment result C3-convertase inhibition.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5fba3e3d-208e-52a0-8fb7-c3bf5097ba5c","evidence_kind":"source_excerpt","locator":"Lines 221-227","start_line":221,"end_line":227,"excerpt":"### ra-c3-convertase-null\n\nRA did not significantly inhibit C3-convertase enzyme activity at concentrations up to 10 mM.\nThis rules against calling the attachment result C3-convertase inhibition.\nModel and exposure: Purified complement system.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Inhibition of complement by covalent attachment of rosmarinic acid to activated C3b. | 1999 | https://pubmed.ncbi.nlm.nih.gov/10353266/ | https://doi.org/10.1016/s0006-2952(99)00044-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Purified complement system.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"606cff22-a6ed-59cd-a84d-87f44c59dc25","stable_key":"import-ca8e9e6a-6953-51ec-b712-2b06398a416d","title":"Rosmarinic acid: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"684b4eab1dc2c0057f679109770af0de9340af5cd933377b590298cf8a752956","revision_id":"4028d9c5-fb17-54c5-b72c-c6d21678a832","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}