{"id":"dc1fd2ad-c17a-5100-9e4e-0ea28c198776","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ampk-activation","predicate":"increases_activity_of","statement":"CGA increased AMPK phosphorylation and measured AMPK activity in L6 myotubes.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"f44ad795-01b4-5d74-97d4-237324e8e8c2","mechanism_event_label":"A cellular energy-sensing pathway responded in cultured rat muscle.","subject":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"object":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"evidence_count":1,"mechanism_event":{"id":"f44ad795-01b4-5d74-97d4-237324e8e8c2","stable_key":"00c24cfe-6189-5244-b81f-5d693e4e177d:chlorogenic_acid-ampk-activation-event","event_type":"biochemical_relationship","label":"A cellular energy-sensing pathway responded in cultured rat muscle.","description":"CGA increased AMPK phosphorylation and measured AMPK activity in L6 myotubes.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"b355feba-1bd3-55d6-9f12-54b9fbadfd58","slug":"rat-slc2a4","display_name":"Rat glucose transporter 4 / Slc2a4","entity_type_key":"protein"},"role":"glucose_transporter","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"705c7f84-5840-55e1-be69-383cc8793a06","slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"378e97ad-f6dc-5b66-a889-e95543049489","slug":"rat-ampk-complexes","display_name":"Rat AMP-activated protein kinase complexes","entity_type_key":"protein_family"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/chlorogenic_acid-research/22412912.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\", \"start_char\": 0, \"end_char\": 1896, \"text_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"chlorogenic-acid","display_name":"Chlorogenic acid / 5-O-caffeoylquinic acid","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Rattus norvegicus","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A cellular energy-sensing pathway responded in cultured rat muscle.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"L6 skeletal-muscle myotubes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"d85da27e-cb44-5f57-9505-2232a927ac52","evidence_kind":"source_excerpt","locator":"Lines 412-423","start_line":412,"end_line":423,"excerpt":"### chlorogenic_acid-ampk-activation\nCGA increased AMPK phosphorylation and measured AMPK activity in L6 myotubes.\nCondition category: normal\nnutrient_topic: Chlorogenic acid research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A cellular energy-sensing pathway responded in cultured rat muscle.\norganism: Rattus norvegicus\ntissue_or_cell_type: L6 skeletal-muscle myotubes\nexperimental_model: L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments\nlimitations: Millimolar culture exposure and injected mouse doses are not dietary human exposures. Phosphorylation and abundance readouts do not by themselves prove every upstream causal step.\nexposure: L6 experiments included 2 mmol/L CGA; mouse acute intervention 250 mg/kg intraperitoneally\nevidence_span: {\"source_cache\": \"artifacts/chlorogenic_acid-research/22412912.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\", \"start_char\": 0, \"end_char\": 1896, \"text_sha256\": \"77c1ccf86d3fb56135a01d2d80b9f1eec3d657e825789f8be490fff93feb8fff\"}\n[chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718","model_system":"L6 myotube signaling with siRNA and pharmacological tests; db/db mouse experiments","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [chlorogenic_acid-p22412912] Chlorogenic acid stimulates glucose transport in skeletal muscle via AMPK activation: a contributor to the beneficial effects of coffee on diabetes. (2012). https://pubmed.ncbi.nlm.nih.gov/22412912/ DOI: 10.1371/journal.pone.0032718","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"98a7cb8d-1d80-5cfe-9690-4068d21878e3","stable_key":"import-00c24cfe-6189-5244-b81f-5d693e4e177d","title":"Chlorogenic acid: metabolism, signaling and nutrient connections (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"dabd56b55fb10d82913b222343d9592d3baf61ef3cc81cf47f3e3e7537eb7992","revision_id":"340aee2f-52a6-56aa-99c1-0a24c534752a","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}