{"id":"dc1f0c6b-07e9-5f8a-b419-a033e035adba","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-human-gldc-plp-site","predicate":"contains-binding-site-for","statement":"Human GLDC cDNA sequencing identified a conserved PLP-binding lysine region corresponding to the chicken enzyme.","claim_class":"identity","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"045423d2-c3c2-59cf-8dd8-e66510001757","mechanism_event_label":"The human enzyme retains the conserved B6-binding machinery.","subject":{"id":"f4497497-e8be-5658-af01-2a75e9802027","slug":"gldc","display_name":"Human glycine decarboxylase / GLDC","entity_type_key":"protein"},"object":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"045423d2-c3c2-59cf-8dd8-e66510001757","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-human-gldc-plp-site-event","event_type":"biochemical_relationship","label":"The human enzyme retains the conserved B6-binding machinery.","description":"Human GLDC cDNA sequencing identified a conserved PLP-binding lysine region corresponding to the chicken enzyme.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"f4497497-e8be-5658-af01-2a75e9802027","slug":"gldc","display_name":"Human glycine decarboxylase / GLDC","entity_type_key":"protein"},"role":"human protein sequence","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"assigned cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Human and chicken cDNA cloning and sequence comparison","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Sequence evidence; human catalytic rates were not established by this cloning study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The human enzyme retains the conserved B6-binding machinery.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-gldc-1991] The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures. (1991). https://pubmed.ncbi.nlm.nih.gov/1993704/ DOI: 10.1016/s0021-9258(18)49991-7","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cloned human GLDC cDNA and deduced protein sequence","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"edbc6f83-73e4-56d2-9b75-909f1eb07e3e","evidence_kind":"source_excerpt","locator":"Lines 671-680","start_line":671,"end_line":680,"excerpt":"### b6-met-human-gldc-plp-site\nHuman GLDC cDNA sequencing identified a conserved PLP-binding lysine region corresponding to the chicken enzyme.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The human enzyme retains the conserved B6-binding machinery.\norganism: Homo sapiens\ntissue_or_cell_type: Cloned human GLDC cDNA and deduced protein sequence\nexperimental_model: Human and chicken cDNA cloning and sequence comparison\nlimitations: Sequence evidence; human catalytic rates were not established by this cloning study.\n[b6-gldc-1991] The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures. (1991). https://pubmed.ncbi.nlm.nih.gov/1993704/ DOI: 10.1016/s0021-9258(18)49991-7","model_system":"Human and chicken cDNA cloning and sequence comparison","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-gldc-1991] The glycine cleavage system. Molecular cloning of the chicken and human glycine decarboxylase cDNAs and some characteristics involved in the deduced protein structures. (1991). https://pubmed.ncbi.nlm.nih.gov/1993704/ DOI: 10.1016/s0021-9258(18)49991-7","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}