{"id":"dbe7f79c-0c24-5aa0-a79f-9ba055af8ba0","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-aldob-variant","predicate":"no_detected_cleavage","statement":"The human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"neutral","is_public":true,"mechanism_event_id":"7d45e67b-37e5-5332-8f60-bf5c2e1ee406","mechanism_event_label":"The human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.","subject":{"id":"6cfe637a-1f43-5b53-ab0b-5e3965c8365d","slug":"human-aldob-r303w","display_name":"Human ALDOB Arg303Trp variant","entity_type_key":"protein_state"},"object":{"id":"a273c7f5-e73a-5464-8947-f2d52f201d88","slug":"fructose-1-phosphate","display_name":"D-Fructose 1-phosphate","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"7d45e67b-37e5-5332-8f60-bf5c2e1ee406","stable_key":"c8c7aa13-14c6-5f5c-9afc-9217d0f2f736:hfcs-aldob-variant-event","event_type":"observed_relationship","label":"The human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.","description":"The human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"6cfe637a-1f43-5b53-ab0b-5e3965c8365d","slug":"human-aldob-r303w","display_name":"Human ALDOB Arg303Trp variant","entity_type_key":"protein_state"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"a273c7f5-e73a-5464-8947-f2d52f201d88","slug":"fructose-1-phosphate","display_name":"D-Fructose 1-phosphate","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"165762fd-033e-58ed-ab15-5b0a43089f43","slug":"aldob","display_name":"Human aldolase B / ALDOB","entity_type_key":"protein"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"availability_state","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null},{"dimension":"dose","value_text":"Wild-type versus hereditary-fructose-intolerance-associated variants; exact substrate series not in abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"duration","value_text":"Assay duration not recovered","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_access","value_text":"Primary abstract/metadata; unrecovered methods explicitly retained.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_scope","value_text":"literature_reviewed; source-specific curation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Recombinant wild-type and Arg303Trp human ALDOB","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_scope","value_text":"Human genetic machinery / fructose component","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"A mutation-specific activity defect is not ordinary dietary intolerance or a population toxicity threshold. Full article is scanned; abstract kinetics used.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"HFCS chapter: actual formulation studies, component biochemistry and interventions are explicitly distinguished.","comparator":null,"unit":null,"notes":"","entity":{"slug":"high-fructose-corn-syrup","display_name":"High-Fructose Corn Syrup / HFCS","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Recombinant wild-type and Arg303Trp human ALDOB","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional and molecular modelling studies of two hereditary fructose intolerance-causing mutations at arginine 303 in human liver aldolase. (2000). https://pubmed.ncbi.nlm.nih.gov/10970798/","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"route","value_text":"In vitro recombinant enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue","value_text":"Fructose-1-phosphate enzyme assay","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"trigger_kind","value_text":"machinery_impairment","comparator":null,"unit":null,"notes":"Imported condition classification; unverified.","entity":null}],"evidence":[{"id":"5e21ca4b-5e0f-5eaa-8539-9b23741c1ec3","evidence_kind":"source_excerpt","locator":"Lines 125-135","start_line":125,"end_line":135,"excerpt":"## hfcs-aldob-variant\nThe human ALDOB Arg303Trp variant had no detectable fructose-1-phosphate activity in the reported assay.\nModel/species: Recombinant wild-type and Arg303Trp human ALDOB\nTissue: Fructose-1-phosphate enzyme assay\nExposure: Wild-type versus hereditary-fructose-intolerance-associated variants; exact substrate series not in abstract\nRoute: In vitro recombinant enzyme assay\nDuration: Assay duration not recovered\nExposure scope: Human genetic machinery / fructose component\nLimits: A mutation-specific activity defect is not ordinary dietary intolerance or a population toxicity threshold. Full article is scanned; abstract kinetics used.\nReference: Functional and molecular modelling studies of two hereditary fructose intolerance-causing mutations at arginine 303 in human liver aldolase. (2000). https://pubmed.ncbi.nlm.nih.gov/10970798/\nAccess: Primary abstract/metadata; unrecovered methods explicitly retained.","model_system":"Recombinant wild-type and Arg303Trp human ALDOB","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact original curation span, not a publisher quotation; provenance retained.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"e863ddf6-7c1e-5a70-8335-7f3ac3eeee92","stable_key":"import-c8c7aa13-14c6-5f5c-9afc-9217d0f2f736","title":"High-Fructose Corn Syrup: mechanism of action and metabolic impact (2026-09-20)","document_type":"imported_text","citation_label":"Original AI-assisted curation of twenty primary studies and official FDA composition information, with one reused canonical glucose-transport claim. Study-specific citations, negative findings and limitations retained. Not publisher full text.","file_path":"","sha256":"f4721923a83b368d549ba110a4f8c8738026210b32f6d29f3bd3200aa420f3b3","revision_id":"45827d73-cb6d-5aff-969b-af364b74b168","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}