{"id":"dbd04a00-080a-5c49-b70d-72db5cbc3787","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:mouse-rct","predicate":"increases_in_recorded_experiment","statement":"C3G consumption promoted macrophage reverse cholesterol transport in the microbiota-dependent ApoE-deficient mouse setting.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"f6f68584-348d-576d-a0b7-add00e49132e","mechanism_event_label":"C3G consumption promoted macrophage reverse cholesterol transport in the microbiota-dependent ApoE-deficient mouse setting.","subject":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"object":{"id":"8fe78323-81db-55e5-a57c-7c5447eb98b9","slug":"mouse-macrophage-reverse-cholesterol-transport","display_name":"Macrophage reverse cholesterol transport in mice","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"f6f68584-348d-576d-a0b7-add00e49132e","stable_key":"35ec55a7-323c-5c28-979e-3bdafe9d5769:mouse-rct-event","event_type":"experimental_observation","label":"C3G consumption promoted macrophage reverse cholesterol transport in the microbiota-dependent ApoE-deficient mouse setting.","description":"**PCA–miR-10b–cholesterol export is a specific experimental branch.** In ApoE-deficient mice, antibiotic depletion and microbiota reacquisition supported microbial involvement in C3G-to-PCA metabolism. PCA reduced macrophage miR-10b; functional tests showed miR-10b repressing ABCA1 and ABCG1 and limiting cholesterol efflux. PCA, unlike C3G at the compared concentrations, increased transporter expression and efflux. C3G feeding promoted macrophage reverse cholesterol transport and lesion regression in the microbiota-dependent mouse setting. The accessible primary abstract reports human- and mouse-derived macrophage experiments but does not fully separate every endpoint by species or list all doses; those remain extraction limits. [Wang et al., 2012](https://pubmed.ncbi.nlm.nih.gov/22821931/).","status":"provisional","compartment":null,"participants":[{"entity":{"id":"75149db0-02f7-5d32-8c7a-5c111d90d8ee","slug":"cyanidin-3-glucoside","display_name":"Cyanidin 3-O-beta-D-glucopyranoside","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"C3G feeding in mice with the relevant microbiota condition","sequence_order":0,"notes":""},{"entity":{"id":"8fe78323-81db-55e5-a57c-7c5447eb98b9","slug":"mouse-macrophage-reverse-cholesterol-transport","display_name":"Macrophage reverse cholesterol transport in mice","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"increase","sequence_order":1,"notes":""},{"entity":{"id":"f7042803-09ec-53d9-9d4c-9a0ce4042c00","slug":"protocatechuic-acid","display_name":"Protocatechuic acid / 3,4-dihydroxybenzoic acid","entity_type_key":"small_molecule"},"role":"microbial product investigated in the study","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary indexed abstract reviewed; full methods, figures, exact doses or endpoint-specific species assignments may remain unextracted.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"C3G feeding in mice with the relevant microbiota condition\", \"comparator\": \"Matched control feeding\", \"endpoint\": \"C3G consumption promoted macrophage reverse cholesterol transport in the microbiota-dependent ApoE-deficient mouse setting.\", \"effect_direction\": \"increase\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; source-derived draft.","entity":null},{"dimension":"experimental_model","value_text":"ApoE-deficient mouse feeding experiment; antibiotics and microbiota reacquisition used to investigate dependence.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Source-derived extraction of a fact-checked reference; access is explicit, not independent raw-data verification.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Diet dose/duration not extracted from accessible abstract. Antibiotics are not selective blockade of a single metabolic step; no human plaque regression inferred.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"C3G consumption promoted macrophage reverse cholesterol transport in the microbiota-dependent ApoE-deficient mouse setting.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Gut microbiota metabolism of anthocyanin promotes reverse cholesterol transport in mice via repressing miRNA-10b. | 2012 | DOI 10.1161/circresaha.112.266502 | PMID 22821931 | https://pubmed.ncbi.nlm.nih.gov/22821931/ | https://doi.org/10.1161/circresaha.112.266502","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference lines 66-66; exact primary location described in quoted passage where extracted.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"17157907-3533-57a4-992b-6e9363fec970","evidence_kind":"source_excerpt","locator":"Lines 66-66","start_line":66,"end_line":66,"excerpt":"**PCA–miR-10b–cholesterol export is a specific experimental branch.** In ApoE-deficient mice, antibiotic depletion and microbiota reacquisition supported microbial involvement in C3G-to-PCA metabolism. PCA reduced macrophage miR-10b; functional tests showed miR-10b repressing ABCA1 and ABCG1 and limiting cholesterol efflux. PCA, unlike C3G at the compared concentrations, increased transporter expression and efflux. C3G feeding promoted macrophage reverse cholesterol transport and lesion regression in the microbiota-dependent mouse setting. The accessible primary abstract reports human- and mouse-derived macrophage experiments but does not fully separate every endpoint by species or list all doses; those remain extraction limits. [Wang et al., 2012](https://pubmed.ncbi.nlm.nih.gov/22821931/).","model_system":"ApoE-deficient mouse feeding experiment; antibiotics and microbiota reacquisition used to investigate dependence.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Exact excerpt of the retained AI-assisted reviewed reference; primary sources are cited in primary_references and access scope is retained. Not a verbatim quotation from a primary paper.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"b08e7f7d-4d34-56d7-a185-2124f8d72b3c","stable_key":"import-35ec55a7-323c-5c28-979e-3bdafe9d5769","title":"Anthocyanins: detailed mechanisms of action (reviewed 4 October 2026)","document_type":"imported_text","citation_label":"Original AI-assisted review of primary studies and, where relevant, official regulatory records. Access level is retained per claim. Corrections, null results and unresolved questions remain explicit. Not publisher full text or independent replication.","file_path":"","sha256":"7cff1a47fbd9c625412b84162b1c823004b4162b7c009f9a11d5807fb8e04ef9","revision_id":"1bd4fa42-bfeb-5148-8250-fc363d8c1de0","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}