{"id":"db6dfb16-8151-5845-972c-15d1db7764ac","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyat-benzoyl","predicate":"catalyzes","statement":"Recombinant human GLYAT variants conjugated glycine to benzoyl-CoA in kinetic assays, forming the glycine-conjugation product hippurate.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"85afa094-18fa-5764-82f0-7b7bae3a0de9","mechanism_event_label":"Activated acyl groups can be transferred onto glycine for disposal.","subject":{"id":"3febb8ae-e5ee-51ef-912f-826f4e2e3ddd","slug":"glyat","display_name":"Human glycine N-acyltransferase / GLYAT","entity_type_key":"protein"},"object":{"id":"eb8bb885-4a0f-5683-89f5-5d4d73902822","slug":"hippuric-acid","display_name":"Hippuric acid / benzoylglycine","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"85afa094-18fa-5764-82f0-7b7bae3a0de9","stable_key":"507ed066-fd6d-5722-9223-97b66302e7e3:glycine-glyat-benzoyl-event","event_type":"observed_relationship","label":"Activated acyl groups can be transferred onto glycine for disposal.","description":"Recombinant human GLYAT variants conjugated glycine to benzoyl-CoA in kinetic assays, forming the glycine-conjugation product hippurate.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"3febb8ae-e5ee-51ef-912f-826f4e2e3ddd","slug":"glyat","display_name":"Human glycine N-acyltransferase / GLYAT","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"eb8bb885-4a0f-5683-89f5-5d4d73902822","slug":"hippuric-acid","display_name":"Hippuric acid / benzoylglycine","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"2b507258-430c-51fe-9fd2-e510c2c197a9","slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"e3f62386-3053-5d49-a6d3-fe9e92a8df63","slug":"benzoyl-coa","display_name":"Benzoyl-CoA","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""},{"entity":{"id":"4faa6456-aff8-59eb-9e3f-3326a436e401","slug":"coenzyme-a","display_name":"Coenzyme A","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":4,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Purified human GLYAT haplotypes, two-substrate kinetic analysis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Reaction uses an activated acyl-CoA, not free benzoate; pathway activation and CoA release remain separate steps.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Glycine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"glycine","display_name":"Glycine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Activated acyl groups can be transferred onto glycine for disposal.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Functional Characterisation of Three Glycine N-Acyltransferase Variants and the Effect on Glycine Conjugation to Benzoyl-CoA. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33803916/ · DOI 10.3390/ijms22063129","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"5fce1113-28a1-59d5-86f9-1a2a4d436549","evidence_kind":"source_excerpt","locator":"Lines 274-280","start_line":274,"end_line":280,"excerpt":"## glycine-glyat-benzoyl\nActivated acyl groups can be transferred onto glycine for disposal.\nRecombinant human GLYAT variants conjugated glycine to benzoyl-CoA in kinetic assays, forming the glycine-conjugation product hippurate.\nModel: Purified human GLYAT haplotypes, two-substrate kinetic analysis.\nLimitations: Reaction uses an activated acyl-CoA, not free benzoate; pathway activation and CoA release remain separate steps.\nEvidence access: Primary full text\nFunctional Characterisation of Three Glycine N-Acyltransferase Variants and the Effect on Glycine Conjugation to Benzoyl-CoA. · 2021 · https://pubmed.ncbi.nlm.nih.gov/33803916/ · DOI 10.3390/ijms22063129","model_system":"Purified human GLYAT haplotypes, two-substrate kinetic analysis.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"7b2859d8-e2bf-51e7-8e32-0715c10cbc49","stable_key":"import-507ed066-fd6d-5722-9223-97b66302e7e3","title":"Glycine: supply, one-carbon allocation, receptors and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. Not publisher full text.","file_path":"","sha256":"4fabd2fc8e71ca156c91023e7340be3f5a02ae03e91d3a24b231c2adfb0de0aa","revision_id":"24801e49-34a8-53c3-bdf5-b59143441450","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}