{"id":"db5da680-a249-5d91-89d1-88e37895fcb3","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-clin-pantethine-mixed-disulfide","predicate":"promotes-formation-of","statement":"Radiolabeled cystine tracing in cystinotic fibroblasts supported formation of cysteamine-cysteine mixed disulfide during pantethine treatment.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"fdb664bd-2f1d-5dd3-9d2c-9ffeae2ee383","mechanism_event_label":"The derivative changed accumulated cystine into a different disulfide product in these patient cells.","subject":{"id":"c0600873-c581-5429-91cb-414f179823ce","slug":"pantethine","display_name":"Pantethine","entity_type_key":"small_molecule"},"object":{"id":"68731353-2609-53b3-a143-1fa2c65a33a7","slug":"cysteamine-cysteine-mixed-disulfide","display_name":"Cysteamine-cysteine mixed disulfide","entity_type_key":"small_molecule"},"evidence_count":1,"mechanism_event":{"id":"fdb664bd-2f1d-5dd3-9d2c-9ffeae2ee383","stable_key":"f992b796-377d-53bf-a39b-7e5d41dbe194:b5-clin-pantethine-mixed-disulfide-event","event_type":"biochemical_relationship","label":"The derivative changed accumulated cystine into a different disulfide product in these patient cells.","description":"Radiolabeled cystine tracing in cystinotic fibroblasts supported formation of cysteamine-cysteine mixed disulfide during pantethine treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"cdd84f4d-3e8f-502a-a8c5-19672cc5346f","slug":"cystine","display_name":"Cystine","entity_type_key":"small_molecule"},"role":"stored sulfur-containing substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"1149e9db-2832-5f4d-aa5b-5aae11bc7788","slug":"cysteamine","display_name":"Cysteamine","entity_type_key":"small_molecule"},"role":"component of mixed disulfide","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"c0600873-c581-5429-91cb-414f179823ce","slug":"pantethine","display_name":"Pantethine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"68731353-2609-53b3-a143-1fa2c65a33a7","slug":"cysteamine-cysteine-mixed-disulfide","display_name":"Cysteamine-cysteine mixed disulfide","entity_type_key":"small_molecule"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary indexed abstract, including metabolite-tracing conclusion.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Radiolabeled cystine metabolite-tracing experiment in cultured patient fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Pantethine or cystamine treatment; specific concentration not retrieved from the primary abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Disease-derived cultured cells; no molecular exporter is assigned without evidence. Formation and efflux of a mixed disulfide do not prove that pantothenate alone treats cystinosis.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"pantothenate","display_name":"Pantothenate (vitamin B5)","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The derivative changed accumulated cystine into a different disulfide product in these patient cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b5-clin-butler1984] Pantethine and cystamine deplete cystine from cystinotic fibroblasts via efflux of cysteamine-cysteine mixed disulfide. (1984). https://pubmed.ncbi.nlm.nih.gov/6746900/ DOI: 10.1172/jci111436","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Cystinotic fibroblast lysosomes and extracellular medium","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"6299c8fc-e4ef-5498-abc1-d843f70378cd","evidence_kind":"source_excerpt","locator":"Lines 1495-1507","start_line":1495,"end_line":1507,"excerpt":"### b5-clin-pantethine-mixed-disulfide\nRadiolabeled cystine tracing in cystinotic fibroblasts supported formation of cysteamine-cysteine mixed disulfide during pantethine treatment.\nCondition category: normal\nnutrient_topic: Pantothenic acid (vitamin B5) research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The derivative changed accumulated cystine into a different disulfide product in these patient cells.\norganism: Homo sapiens\ntissue_or_cell_type: Cystinotic fibroblast lysosomes and extracellular medium\nexperimental_model: Radiolabeled cystine metabolite-tracing experiment in cultured patient fibroblasts\nlimitations: Disease-derived cultured cells; no molecular exporter is assigned without evidence. Formation and efflux of a mixed disulfide do not prove that pantothenate alone treats cystinosis.\nexposure: Pantethine or cystamine treatment; specific concentration not retrieved from the primary abstract.\ncross_nutrient: false\nevidence_location: Primary indexed abstract, including metabolite-tracing conclusion.\n[b5-clin-butler1984] Pantethine and cystamine deplete cystine from cystinotic fibroblasts via efflux of cysteamine-cysteine mixed disulfide. (1984). https://pubmed.ncbi.nlm.nih.gov/6746900/ DOI: 10.1172/jci111436","model_system":"Radiolabeled cystine metabolite-tracing experiment in cultured patient fibroblasts","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b5-clin-butler1984] Pantethine and cystamine deplete cystine from cystinotic fibroblasts via efflux of cysteamine-cysteine mixed disulfide. (1984). https://pubmed.ncbi.nlm.nih.gov/6746900/ DOI: 10.1172/jci111436","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"0716b500-dd0b-5425-b9d0-d88bc9c09e86","stable_key":"import-f992b796-377d-53bf-a39b-7e5d41dbe194","title":"Pantothenic acid (vitamin B5): coenzyme A, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"b5a49f5a2373ca6064c9a4e014e052dad2f6f199a82bb09b26b82b831c36110b","revision_id":"78f3e793-f084-5ee2-8703-b661c4b650bf","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}