{"id":"db1d79c5-702d-506a-9bf6-7305ea740a1c","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-arv1-pi","predicate":"enhances_pi_utilization_by","statement":"ARV1-containing GPI-GnT used PI more efficiently than the ARV1-less complex in vitro.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"90008e60-81b1-5591-975d-4e40a2b9a3b7","mechanism_event_label":"The anchor pathway depends on substrate handling, not only the total amount of inositol.","subject":{"id":"4b3dc3af-3707-5795-aae3-8383c7fe2de7","slug":"arv1","display_name":"ARV1 (human ER membrane protein)","entity_type_key":"protein"},"object":{"id":"9d901059-e0d6-5670-a99b-5276cd5c7b64","slug":"gpi-gnt-complex","display_name":"Human GPI N-acetylglucosaminyltransferase complex","entity_type_key":"protein_complex"},"evidence_count":1,"mechanism_event":{"id":"90008e60-81b1-5591-975d-4e40a2b9a3b7","stable_key":"e69ef6a0-0e23-5eac-aca1-cf333781b962:ino-arv1-pi-event","event_type":"biochemical_relationship","label":"The anchor pathway depends on substrate handling, not only the total amount of inositol.","description":"ARV1-containing GPI-GnT used PI more efficiently than the ARV1-less complex in vitro.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"ae758982-4634-5f3a-b9c7-dbc58b744484","slug":"phosphatidylinositol","display_name":"Phosphatidylinositol (PI)","entity_type_key":"lipid"},"role":"substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4b3dc3af-3707-5795-aae3-8383c7fe2de7","slug":"arv1","display_name":"ARV1 (human ER membrane protein)","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"9d901059-e0d6-5670-a99b-5276cd5c7b64","slug":"gpi-gnt-complex","display_name":"Human GPI N-acetylglucosaminyltransferase complex","entity_type_key":"protein_complex"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/inositol-research/40378954.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"858f4bd2342d09239e21945d3d83aab06d84f33da26cb788e3ac091b713a1917\", \"start_char\": 0, \"end_char\": 1260, \"text_sha256\": \"858f4bd2342d09239e21945d3d83aab06d84f33da26cb788e3ac091b713a1917\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human-cell gene perturbation and isolated-complex activity","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"ARV1/PIGQ association and PI utilization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"The ring-shaped assembly was AlphaFold-predicted, not an experimentally resolved structure. Effects on GPI-anchored proteins varied by cell and protein.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Inositol research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"inositol","display_name":"Inositol (stereoisomer family)","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The anchor pathway depends on substrate handling, not only the total amount of inositol.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Fibroblasts, HEK293 cells and ER enzyme complex","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"306aeac8-cbb6-5ec1-bcd4-294dacc36699","evidence_kind":"source_excerpt","locator":"Lines 1146-1157","start_line":1146,"end_line":1157,"excerpt":"### ino-arv1-pi\nARV1-containing GPI-GnT used PI more efficiently than the ARV1-less complex in vitro.\nCondition category: normal\nnutrient_topic: Inositol research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The anchor pathway depends on substrate handling, not only the total amount of inositol.\norganism: Homo sapiens\ntissue_or_cell_type: Fibroblasts, HEK293 cells and ER enzyme complex\nexperimental_model: Human-cell gene perturbation and isolated-complex activity\nlimitations: The ring-shaped assembly was AlphaFold-predicted, not an experimentally resolved structure. Effects on GPI-anchored proteins varied by cell and protein.\nexposure: ARV1/PIGQ association and PI utilization\nevidence_span: {\"source_cache\": \"artifacts/inositol-research/40378954.abstract.txt\", \"locator\": \"Exact primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"858f4bd2342d09239e21945d3d83aab06d84f33da26cb788e3ac091b713a1917\", \"start_char\": 0, \"end_char\": 1260, \"text_sha256\": \"858f4bd2342d09239e21945d3d83aab06d84f33da26cb788e3ac091b713a1917\"}\n[ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","model_system":"Human-cell gene perturbation and isolated-complex activity","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ino-p40378954] ARV1 is a component of the enzyme initiating glycosylphosphatidylinositol biosynthesis. (2025). https://pubmed.ncbi.nlm.nih.gov/40378954/ DOI: 10.1016/j.jbc.2025.110236","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"d590c659-b774-50e6-9f1d-aff61d5f7c9c","stable_key":"import-e69ef6a0-0e23-5eac-aca1-cf333781b962","title":"Inositol: synthesis, signaling, mineral interactions and conditional deficiency (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"f7adbcedad647d5f8a3cc9e46bfa515e83c508be9c2a4e9d59c5f5dfb0667d53","revision_id":"a82d187c-60fb-52d1-8482-eadb92860f33","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}