{"id":"daa050a8-d0a6-5c1a-8bed-b2033345a6ee","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-sasp-conditioned-medium","predicate":"decreases_in_recorded_comparison","statement":"Conditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.","claim_class":"observational","status":"source_recorded","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"d06bc2bf-44e3-5c38-8d40-d5fea09501b5","mechanism_event_label":"Conditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.","subject":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"object":{"id":"dec89c2d-66d8-577e-8302-d8b99dc7c087","slug":"api-sasp-conditioned-cancer-response","display_name":"Breast-cancer-cell response to medium from apigenin-treated senescent fibroblasts","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d06bc2bf-44e3-5c38-8d40-d5fea09501b5","stable_key":"ff370329-68a3-573d-96ee-597de78a3967:api-sasp-conditioned-medium-event","event_type":"experimental_observation","label":"Conditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.","description":"### api-sasp-conditioned-medium\n\nConditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.\nIndirect conditioned-medium effects must not be relabelled direct cancer-cell apigenin exposure.\nModel and exposure: Human fibroblast-to-cancer-cell conditioned-medium comparison.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. | 2017 | https://pubmed.ncbi.nlm.nih.gov/28378188/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC5411372/ | https://doi.org/10.1007/s11357-017-9970-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"4425961f-4764-56d3-9887-87e47749379b","slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"},"role":"tested factor","stoichiometry":null,"state_label":"Medium conditioned by apigenin-treated senescent fibroblasts","sequence_order":0,"notes":""},{"entity":{"id":"dec89c2d-66d8-577e-8302-d8b99dc7c087","slug":"api-sasp-conditioned-cancer-response","display_name":"Breast-cancer-cell response to medium from apigenin-treated senescent fibroblasts","entity_type_key":"cellular_process"},"role":"measured outcome","stoichiometry":null,"state_label":"decrease","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_contrast","value_text":"{\"intervention\": \"Medium conditioned by apigenin-treated senescent fibroblasts\", \"comparator\": \"Medium from untreated senescent fibroblasts\", \"endpoint\": \"Conditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.\", \"effect_direction\": \"decrease\", \"combination\": \"single\", \"conditions\": []}","comparator":null,"unit":null,"notes":"Explicit extracted experimental comparison; recorded from source.","entity":null},{"dimension":"experimental_model","value_text":"Human fibroblast-to-cancer-cell conditioned-medium comparison.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"interpretation_status","value_text":"Recorded from source with stated access limits; ungraded, not independent replication.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Apigenin collection; exact intervention and molecular form govern interpretation.","comparator":null,"unit":null,"notes":"","entity":{"slug":"apigenin","display_name":"Apigenin","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"Conditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. | https://pubmed.ncbi.nlm.nih.gov/28378188/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC5411372/ | https://doi.org/10.1007/s11357-017-9970-1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"source_locator","value_text":"Reviewed reference api-sasp-conditioned-medium; Indirect conditioned-medium effects must not be relabelled direct cancer-cell apigenin exposure.","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"be7ff78f-be30-5841-8ec4-194c0e3b7f6e","evidence_kind":"source_excerpt","locator":"Lines 760-766","start_line":760,"end_line":766,"excerpt":"### api-sasp-conditioned-medium\n\nConditioned medium from apigenin-treated senescent fibroblasts showed less pro-aggressive activity toward the breast-cancer cells in the study.\nIndirect conditioned-medium effects must not be relabelled direct cancer-cell apigenin exposure.\nModel and exposure: Human fibroblast-to-cancer-cell conditioned-medium comparison.\nLimitations: Limited to the recorded preparation, model, exposure and endpoint. Does not establish a human dietary deficiency, clinical benefit or unique molecular mediation.\nReference: Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells. | 2017 | https://pubmed.ncbi.nlm.nih.gov/28378188/ | https://pmc.ncbi.nlm.nih.gov/articles/PMC5411372/ | https://doi.org/10.1007/s11357-017-9970-1 | Access: Indexed primary abstract/metadata only; unextracted doses, figures and methods remain unknown.","model_system":"Human fibroblast-to-cancer-cell conditioned-medium comparison.","directness":"reported_statement","verification_status":"source_recorded","notes":"Original paraphrase in the retained reviewed reference, not a verbatim primary-paper quotation. Access level is explicit.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"54eac90b-add1-5f56-9036-d900307c5697","stable_key":"import-ff370329-68a3-573d-96ee-597de78a3967","title":"Apigenin: detailed mechanisms of action (reviewed 10 October 2026)","document_type":"imported_text","citation_label":"AI-assisted original curation of primary studies; claim-local access and experimental limitations stated. Includes original-draft correction history. No independent replication claimed.","file_path":"","sha256":"ede6e5c6b91d66b66844f2ff667a7f8303d9ac1281f15dc09db703f7de4da942","revision_id":"b41a39a2-0197-549c-97a5-1765e6696931","review_status":"source_recorded","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}