{"id":"da7135b7-aa06-5b32-8cbd-5898565fae17","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-feedback","predicate":"inhibits","statement":"Hypoiodite occupied the distal heme cavity; ABTS activity experiments supported product inhibition.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"negative","is_public":true,"mechanism_event_id":"b242bc0e-ddcb-5969-9c4c-45c95e59b2ca","mechanism_event_label":"The enzyme product can limit further enzyme activity.","subject":{"id":"86130da0-319f-5245-b339-705bb336d517","slug":"hypoiodite-ion","display_name":"Hypoiodite ion (OI−)","entity_type_key":"ion"},"object":{"id":"ae57b6a5-04e5-512c-bdad-87430178a058","slug":"lpo-catalytic-activity","display_name":"Lactoperoxidase catalytic activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"b242bc0e-ddcb-5969-9c4c-45c95e59b2ca","stable_key":"79018983-9179-5c1a-8e7a-d6f47a13582b:ki-lpo-feedback-event","event_type":"observed_relationship","label":"The enzyme product can limit further enzyme activity.","description":"Hypoiodite occupied the distal heme cavity; ABTS activity experiments supported product inhibition.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"86130da0-319f-5245-b339-705bb336d517","slug":"hypoiodite-ion","display_name":"Hypoiodite ion (OI−)","entity_type_key":"ion"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ae57b6a5-04e5-512c-bdad-87430178a058","slug":"lpo-catalytic-activity","display_name":"Lactoperoxidase catalytic activity","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"3d671567-4fb6-5b5f-a328-156987d5b396","slug":"lactoperoxidase-family","display_name":"Lactoperoxidase enzyme family","entity_type_key":"protein_family"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified LPO crystallography and activity assays; ammonium iodide supplied the anion, not KI.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure_category","value_text":"Study-specific exposure, including pharmacological and in-vitro conditions; normal is the schema fallback outside the three availability categories.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Structural/biochemical model; enzyme species not assigned from abstract. Cofactor chemistry does not establish dietary iron responsiveness.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Potassium iodide research collection; shared-anion and comparator studies are not all KI interventions.","comparator":null,"unit":null,"notes":"","entity":{"slug":"potassium-iodide","display_name":"Potassium iodide","entity_type_key":"chemical_species"}},{"dimension":"plain_language","value_text":"The enzyme product can limit further enzyme activity.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Structural evidence of the oxidation of iodide ion into hyper-reactive hypoiodite ion by mammalian heme lactoperoxidase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34761444/ · DOI 10.1002/pro.4230","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"f85f3720-b63e-5d13-a1d8-534f9a50b128","evidence_kind":"source_excerpt","locator":"Lines 240-246","start_line":240,"end_line":246,"excerpt":"## ki-lpo-feedback\nThe enzyme product can limit further enzyme activity.\nHypoiodite occupied the distal heme cavity; ABTS activity experiments supported product inhibition.\nModel: Purified LPO crystallography and activity assays; ammonium iodide supplied the anion, not KI.\nLimitations: Structural/biochemical model; enzyme species not assigned from abstract. Cofactor chemistry does not establish dietary iron responsiveness.\nEvidence location: Primary abstract\nStructural evidence of the oxidation of iodide ion into hyper-reactive hypoiodite ion by mammalian heme lactoperoxidase. · 2022 · https://pubmed.ncbi.nlm.nih.gov/34761444/ · DOI 10.1002/pro.4230","model_system":"Purified LPO crystallography and activity assays; ammonium iodide supplied the anion, not KI.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Curation paraphrase; primary evidence location and source kind retained above.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"dc3ce411-f68f-5eb8-87c1-1592665f9cb6","stable_key":"import-79018983-9179-5c1a-8e7a-d6f47a13582b","title":"Potassium iodide: thyroid and non-thyroid mechanisms, interactions and discovery questions (2026-09-18)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary-study references, chemical references and label statements individually identified. 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