{"id":"da3e30b4-990f-5baa-82da-0e664257c3a3","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-psc-exchanger","predicate":"contributes_to_exchange","statement":"SLC1A4 perturbation and isotope-flux studies supported a role for SLC1A4, alongside other transporters, in rapid alanine exchange by human pancreatic stellate cells.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"62ac3add-d76f-585b-8b3b-d331f6349ead","mechanism_event_label":"An exchanger helps neighboring cells share alanine.","subject":{"id":"5a802528-9866-5200-89fe-d32e234882e1","slug":"slc1a4","display_name":"Human neutral amino acid exchanger ASCT1 / SLC1A4","entity_type_key":"protein"},"object":{"id":"5584b5d8-44b3-5e0c-8451-462fd5b44205","slug":"human-psc-alanine-secretion","display_name":"Alanine secretion from human pancreatic stellate cells","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"62ac3add-d76f-585b-8b3b-d331f6349ead","stable_key":"10aa417f-4b03-599f-a453-4aa09edeb27c:alanine-psc-exchanger-event","event_type":"observed_relationship","label":"An exchanger helps neighboring cells share alanine.","description":"SLC1A4 perturbation and isotope-flux studies supported a role for SLC1A4, alongside other transporters, in rapid alanine exchange by human pancreatic stellate cells.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"5a802528-9866-5200-89fe-d32e234882e1","slug":"slc1a4","display_name":"Human neutral amino acid exchanger ASCT1 / SLC1A4","entity_type_key":"protein"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"5584b5d8-44b3-5e0c-8451-462fd5b44205","slug":"human-psc-alanine-secretion","display_name":"Alanine secretion from human pancreatic stellate cells","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"ebdc4461-c059-563e-88b0-422c21fdaa25","slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""},{"entity":{"id":"38de8704-84db-5770-ac1d-242cd787e798","slug":"sodium-ion","display_name":"Sodium ion","entity_type_key":"ion"},"role":"context_participant","stoichiometry":null,"state_label":"","sequence_order":3,"notes":""}]},"contexts":[{"dimension":"evidence_access","value_text":"Primary full text","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Human PSC transporter and flux experiments.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"SLC1A4 was not the sole alanine exporter and exchange is not equivalent to an irreversible one-way pump.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"L-Alanine collection; species, compartment, exposure, co-substrates and manipulation remain explicit.","comparator":null,"unit":null,"notes":"","entity":{"slug":"alanine","display_name":"L-Alanine","entity_type_key":"small_molecule"}},{"dimension":"plain_language","value_text":"An exchanger helps neighboring cells share alanine.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"Selective Alanine Transporter Utilization Creates a Targetable Metabolic Niche in Pancreatic Cancer. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32341021/ · DOI 10.1158/2159-8290.CD-19-0959","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"20143f85-bf45-5c00-a2d5-d750b18cd6f0","evidence_kind":"source_excerpt","locator":"Lines 176-182","start_line":176,"end_line":182,"excerpt":"## alanine-psc-exchanger\nAn exchanger helps neighboring cells share alanine.\nSLC1A4 perturbation and isotope-flux studies supported a role for SLC1A4, alongside other transporters, in rapid alanine exchange by human pancreatic stellate cells.\nModel: Human PSC transporter and flux experiments.\nLimitations: SLC1A4 was not the sole alanine exporter and exchange is not equivalent to an irreversible one-way pump.\nEvidence access: Primary full text\nSelective Alanine Transporter Utilization Creates a Targetable Metabolic Niche in Pancreatic Cancer. · 2020 · https://pubmed.ncbi.nlm.nih.gov/32341021/ · DOI 10.1158/2159-8290.CD-19-0959","model_system":"Human PSC transporter and flux experiments.","directness":"reported_statement","verification_status":"source_derived_draft","notes":"Original curation paraphrase; evidence access and experimental limitations specified.","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"01c5554c-a8ba-5c86-9b01-a0fa1a886cdb","stable_key":"import-10aa417f-4b03-599f-a453-4aa09edeb27c","title":"L-Alanine: carbon, nitrogen, protein synthesis and cross-nutrient mechanisms (2026-09-19)","document_type":"imported_text","citation_label":"AI-assisted research curation; primary references, access levels and experimental limitations individually identified. 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