{"id":"da2fa408-b567-54bd-b4a8-8b39cee3f3f0","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-tocopheroxyl-detection","predicate":"forms-radical-intermediate","statement":"Electron paramagnetic resonance detected alpha-tocopheroxyl radical at approximately 10^-8–10^-7 M during Fe(III)-complex-initiated oxidation of soybean phosphatidylcholine liposomes containing alpha-tocopherol.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"3d21d6be-fecd-500c-bd7f-e385c631f614","mechanism_event_label":"The experiment directly observed the vitamin E radical intermediate inside an artificial membrane.","subject":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"object":{"id":"ec585435-0fdf-5f6f-97d9-6fff230d405e","slug":"alpha-tocopheroxyl-radical","display_name":"Alpha-tocopheroxyl radical","entity_type_key":"chemical_species"},"evidence_count":1,"mechanism_event":{"id":"3d21d6be-fecd-500c-bd7f-e385c631f614","stable_key":"e0ea2d6a-7429-5e9f-b774-41e5e3288da3:ver-tocopheroxyl-detection-event","event_type":"biochemical_relationship","label":"The experiment directly observed the vitamin E radical intermediate inside an artificial membrane.","description":"Electron paramagnetic resonance detected alpha-tocopheroxyl radical at approximately 10^-8–10^-7 M during Fe(III)-complex-initiated oxidation of soybean phosphatidylcholine liposomes containing alpha-tocopherol.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"40a6525a-76b8-576e-b73d-1e11aecf5f8d","slug":"alpha-tocopherol","display_name":"Alpha-tocopherol","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"ec585435-0fdf-5f6f-97d9-6fff230d405e","slug":"alpha-tocopheroxyl-radical","display_name":"Alpha-tocopheroxyl radical","entity_type_key":"chemical_species"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"false","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"evidence_location","value_text":"Primary abstract","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"EPR with optical and oxygen-consumption measurements","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Fe(III)-triethylenetetramine initiator.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Artificial membrane system; does not quantify in vivo radical abundance.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin E research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-e","display_name":"Vitamin E","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Cell-free soybean lipid preparation","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"The experiment directly observed the vitamin E radical intermediate inside an artificial membrane.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[ver-scarpa1984-reuse] Formation of alpha-tocopherol radical and recycling of alpha-tocopherol by ascorbate during peroxidation of phosphatidylcholine liposomes. An electron paramagnetic resonance study. (1984). https://pubmed.ncbi.nlm.nih.gov/6089911/ DOI: 10.1016/0304-4165(84)90070-9","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Phosphatidylcholine liposomes","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"fedfaa85-b682-5869-b7e7-b2d119c64132","evidence_kind":"source_excerpt","locator":"Lines 494-506","start_line":494,"end_line":506,"excerpt":"### ver-tocopheroxyl-detection\nElectron paramagnetic resonance detected alpha-tocopheroxyl radical at approximately 10^-8–10^-7 M during Fe(III)-complex-initiated oxidation of soybean phosphatidylcholine liposomes containing alpha-tocopherol.\nCondition category: normal\nnutrient_topic: Vitamin E research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: The experiment directly observed the vitamin E radical intermediate inside an artificial membrane.\norganism: Cell-free soybean lipid preparation\ntissue_or_cell_type: Phosphatidylcholine liposomes\nexperimental_model: EPR with optical and oxygen-consumption measurements\nlimitations: Artificial membrane system; does not quantify in vivo radical abundance.\nexposure: Fe(III)-triethylenetetramine initiator.\ncross_nutrient: false\nevidence_location: Primary abstract\n[ver-scarpa1984-reuse] Formation of alpha-tocopherol radical and recycling of alpha-tocopherol by ascorbate during peroxidation of phosphatidylcholine liposomes. An electron paramagnetic resonance study. (1984). https://pubmed.ncbi.nlm.nih.gov/6089911/ DOI: 10.1016/0304-4165(84)90070-9","model_system":"EPR with optical and oxygen-consumption measurements","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [ver-scarpa1984-reuse] Formation of alpha-tocopherol radical and recycling of alpha-tocopherol by ascorbate during peroxidation of phosphatidylcholine liposomes. An electron paramagnetic resonance study. (1984). https://pubmed.ncbi.nlm.nih.gov/6089911/ DOI: 10.1016/0304-4165(84)90070-9","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"3ac41dba-4727-5116-a556-ac3bbb0dc41c","stable_key":"import-e0ea2d6a-7429-5e9f-b774-41e5e3288da3","title":"Vitamin E: transport, membrane protection and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"a90b6565e092c6a2da07f74cf758792c2cf63b40746260c8ff12bd64322c36f4","revision_id":"956793d1-23e4-5397-9291-25c1732a8e02","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}