{"id":"da01c794-3b9f-5a8c-9f0a-9e096f950bb4","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-glut1-activation","predicate":"increases_transport_activity_of","statement":"Berberine acutely increased GLUT1-mediated uptake in L929 cells and decreased apparent glucose-uptake Km without changing Vmax.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"context_dependent","is_public":true,"mechanism_event_id":"d486e00d-b34a-5e68-b44e-4079a82de74f","mechanism_event_label":"A transporter can move glucose differently without the cell making more transporters.","subject":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"object":{"id":"4a8cf254-a1c3-551f-a01e-9d8aeec5b771","slug":"mouse-slc2a1","display_name":"Mouse glucose transporter GLUT1 / Slc2a1","entity_type_key":"protein"},"evidence_count":1,"mechanism_event":{"id":"d486e00d-b34a-5e68-b44e-4079a82de74f","stable_key":"7484635e-cc8f-5cd6-a978-6dc5775a7db5:berberine-glut1-activation-event","event_type":"biochemical_relationship","label":"A transporter can move glucose differently without the cell making more transporters.","description":"Berberine acutely increased GLUT1-mediated uptake in L929 cells and decreased apparent glucose-uptake Km without changing Vmax.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"8ae7848b-f172-5e09-8acf-5ca914907b0b","slug":"glucose","display_name":"D-glucose","entity_type_key":"small_molecule"},"role":"transported_substrate","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"7bbe0633-b2f1-510f-adc0-3c4d0ddad05d","slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"},"role":"subject","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"4a8cf254-a1c3-551f-a01e-9d8aeec5b771","slug":"mouse-slc2a1","display_name":"Mouse glucose transporter GLUT1 / Slc2a1","entity_type_key":"protein"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"evidence_span","value_text":"{\"source_cache\": \"artifacts/berberine-research/21545824.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"18e315a501ae688fc71a7761667e58ae71fdd4b3fae19b7ef0f83889dbae2825\", \"start_char\": 0, \"end_char\": 1865, \"text_sha256\": \"18e315a501ae688fc71a7761667e58ae71fdd4b3fae19b7ef0f83889dbae2825\"}","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Acute glucose-uptake kinetics and kinase inhibitors","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Minutes of berberine exposure; maximum stimulation above 40 micromolar","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Single-cell model and high concentrations; kinase inhibitors are not fully selective. No inference of a matching human glucose-lowering magnitude.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Berberine research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"berberine","display_name":"Berberine","entity_type_key":"small_molecule"}},{"dimension":"organism","value_text":"Mouse L929 fibroblasts expressing GLUT1","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"A transporter can move glucose differently without the cell making more transporters.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[berberine-p21545824] Berberine acutely activates the glucose transport activity of GLUT1. (2011). https://pubmed.ncbi.nlm.nih.gov/21545824/ DOI: 10.1016/j.biochi.2011.04.013","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"GLUT1-mediated glucose uptake","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"87620483-cac8-5843-a7df-7270402e56c7","evidence_kind":"source_excerpt","locator":"Lines 519-530","start_line":519,"end_line":530,"excerpt":"### berberine-glut1-activation\nBerberine acutely increased GLUT1-mediated uptake in L929 cells and decreased apparent glucose-uptake Km without changing Vmax.\nCondition category: normal\nnutrient_topic: Berberine research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: A transporter can move glucose differently without the cell making more transporters.\norganism: Mouse L929 fibroblasts expressing GLUT1\ntissue_or_cell_type: GLUT1-mediated glucose uptake\nexperimental_model: Acute glucose-uptake kinetics and kinase inhibitors\nlimitations: Single-cell model and high concentrations; kinase inhibitors are not fully selective. No inference of a matching human glucose-lowering magnitude.\nexposure: Minutes of berberine exposure; maximum stimulation above 40 micromolar\nevidence_span: {\"source_cache\": \"artifacts/berberine-research/21545824.abstract.txt\", \"locator\": \"Primary indexed abstract; zero-based, end-exclusive Unicode character offsets\", \"file_sha256\": \"18e315a501ae688fc71a7761667e58ae71fdd4b3fae19b7ef0f83889dbae2825\", \"start_char\": 0, \"end_char\": 1865, \"text_sha256\": \"18e315a501ae688fc71a7761667e58ae71fdd4b3fae19b7ef0f83889dbae2825\"}\n[berberine-p21545824] Berberine acutely activates the glucose transport activity of GLUT1. (2011). https://pubmed.ncbi.nlm.nih.gov/21545824/ DOI: 10.1016/j.biochi.2011.04.013","model_system":"Acute glucose-uptake kinetics and kinase inhibitors","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [berberine-p21545824] Berberine acutely activates the glucose transport activity of GLUT1. (2011). https://pubmed.ncbi.nlm.nih.gov/21545824/ DOI: 10.1016/j.biochi.2011.04.013","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"9bb61e1d-e5cf-5b05-a9ca-409291f7a313","stable_key":"import-7484635e-cc8f-5cd6-a978-6dc5775a7db5","title":"Berberine: metabolism, nutrient connections and drug interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"2f376a035ae79c93e62e2ea4a8d5385643bf16553f108d12b6a80c89e6d41f4c","revision_id":"2a099429-1c4a-55be-aa2c-0565d08bda80","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}