{"id":"d9c7c52c-e673-5121-bf63-8be60084792a","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-fibroblast-lysyl-activity","predicate":"increases","statement":"Human skin fibroblast lysyl hydroxylase activity increased approximately threefold after the study ascorbate treatment.","claim_class":"observational","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"d7aa2c45-d7d8-551b-80c2-80bcd5afa9db","mechanism_event_label":"Vitamin C also changed the amount of lysyl hydroxylase activity measured in these cells.","subject":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"object":{"id":"4cbcce85-510e-50f7-a837-c0ceaf1d69ce","slug":"human-fibroblast-lysyl-hydroxylase-activity","display_name":"Human fibroblast lysyl hydroxylase activity","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"d7aa2c45-d7d8-551b-80c2-80bcd5afa9db","stable_key":"cb568d28-484a-5c2e-9fcc-2d780358e514:vc-enzyme-fibroblast-lysyl-activity-event","event_type":"observed_intervention","label":"Vitamin C also changed the amount of lysyl hydroxylase activity measured in these cells.","description":"Human skin fibroblast lysyl hydroxylase activity increased approximately threefold after the study ascorbate treatment.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"38d0c4d9-53d1-5239-bfe3-c1b5e9b79085","slug":"ascorbate","display_name":"L-Ascorbate","entity_type_key":"small_molecule"},"role":"supporting-reductant","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"4cbcce85-510e-50f7-a837-c0ceaf1d69ce","slug":"human-fibroblast-lysyl-hydroxylase-activity","display_name":"Human fibroblast lysyl hydroxylase activity","entity_type_key":"cellular_process"},"role":"readout","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"a11a1a13-9184-57b3-b5bf-45708f4c3227","slug":"lysyl-hydroxylases","display_name":"Collagen lysyl hydroxylase family","entity_type_key":"protein_family"},"role":"enzyme-family","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"cross_nutrient","value_text":"Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"experimental_model","value_text":"Cultured human skin fibroblasts","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"exposure","value_text":"Prolonged ascorbate exposure versus no added ascorbate; exact culture concentration not verified in accessible abstract.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"Activity assay does not resolve human PLOD isoforms or distinguish all possible expression/stability effects.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin C research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-c","display_name":"Vitamin C","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Vitamin C also changed the amount of lysyl hydroxylase activity measured in these cells.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[collagen1981] Regulation of collagen synthesis by ascorbic acid. (1981). https://pubmed.ncbi.nlm.nih.gov/6265920/ DOI: 10.1073/pnas.78.5.2879","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Skin fibroblasts","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"aca169bb-93f5-5eb2-bff2-7dd4d697f1d2","evidence_kind":"source_excerpt","locator":"Lines 663-674","start_line":663,"end_line":674,"excerpt":"### vc-enzyme-fibroblast-lysyl-activity\nHuman skin fibroblast lysyl hydroxylase activity increased approximately threefold after the study ascorbate treatment.\nCondition category: normal\nnutrient_topic: Vitamin C research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Vitamin C also changed the amount of lysyl hydroxylase activity measured in these cells.\norganism: Homo sapiens\ntissue_or_cell_type: Skin fibroblasts\nexperimental_model: Cultured human skin fibroblasts\nlimitations: Activity assay does not resolve human PLOD isoforms or distinguish all possible expression/stability effects.\ncross_nutrient: Vitamin C chemistry in collagen, modified-lysine/carnitine metabolism or copper-dependent peptide/catecholamine processing.\nexposure: Prolonged ascorbate exposure versus no added ascorbate; exact culture concentration not verified in accessible abstract.\n[collagen1981] Regulation of collagen synthesis by ascorbic acid. 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