{"id":"d98a2db7-bb53-5564-8b6e-bbf06e06e9ba","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-shmt2-assembly","predicate":"promotes","statement":"Adding PLP shifted purified human SHMT2 from dimers toward tetramers.","claim_class":"mechanistic","status":"source_derived_draft","evidence_grade":"ungraded","direction":"positive","is_public":true,"mechanism_event_id":"70b04361-9dcb-5ec9-a9bf-bd9794ebd566","mechanism_event_label":"Active B6 also helps assemble SHMT2.","subject":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"object":{"id":"fb680e6b-c175-5345-a011-f509ac5f011e","slug":"shmt2-tetramer-assembly","display_name":"SHMT2 tetramer assembly","entity_type_key":"cellular_process"},"evidence_count":1,"mechanism_event":{"id":"70b04361-9dcb-5ec9-a9bf-bd9794ebd566","stable_key":"1310afbd-6010-586e-805d-551d846da421:b6-met-shmt2-assembly-event","event_type":"biochemical_relationship","label":"Active B6 also helps assemble SHMT2.","description":"Adding PLP shifted purified human SHMT2 from dimers toward tetramers.","status":"provisional","compartment":null,"participants":[{"entity":{"id":"2559e210-62e5-5cd8-bc77-538a9aecd7c8","slug":"shmt2","display_name":"SHMT2","entity_type_key":"protein"},"role":"enzyme","stoichiometry":null,"state_label":"","sequence_order":0,"notes":""},{"entity":{"id":"6b656ff5-9532-5da4-8eea-8ca163a48649","slug":"pyridoxal-phosphate","display_name":"PLP","entity_type_key":"small_molecule"},"role":"added cofactor","stoichiometry":null,"state_label":"","sequence_order":1,"notes":""},{"entity":{"id":"fb680e6b-c175-5345-a011-f509ac5f011e","slug":"shmt2-tetramer-assembly","display_name":"SHMT2 tetramer assembly","entity_type_key":"cellular_process"},"role":"target","stoichiometry":null,"state_label":"","sequence_order":2,"notes":""}]},"contexts":[{"dimension":"experimental_model","value_text":"Purified human SHMT1 and SHMT2; structures and solution oligomerization","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"limitations","value_text":"In-vitro cofactor titration; no dietary deficiency threshold was tested.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"nutrient_topic","value_text":"Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.","comparator":null,"unit":null,"notes":"","entity":{"slug":"vitamin-b6","display_name":"Vitamin B6","entity_type_key":"chemical_species"}},{"dimension":"organism","value_text":"Homo sapiens","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"plain_language","value_text":"Active B6 also helps assemble SHMT2.","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"primary_references","value_text":"[b6-shmt-2015] How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state (2015). https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.13211 DOI: 10.1111/febs.13211","comparator":null,"unit":null,"notes":"","entity":null},{"dimension":"tissue_or_cell_type","value_text":"Purified recombinant protein; no intact tissue","comparator":null,"unit":null,"notes":"","entity":null}],"evidence":[{"id":"da731f52-eae8-5863-924e-f65b02029691","evidence_kind":"source_excerpt","locator":"Lines 626-635","start_line":626,"end_line":635,"excerpt":"### b6-met-shmt2-assembly\nAdding PLP shifted purified human SHMT2 from dimers toward tetramers.\nCondition category: normal\nnutrient_topic: Vitamin B6 research collection; topical membership is not evidence of a direct dietary effect.\nplain_language: Active B6 also helps assemble SHMT2.\norganism: Homo sapiens\ntissue_or_cell_type: Purified recombinant protein; no intact tissue\nexperimental_model: Purified human SHMT1 and SHMT2; structures and solution oligomerization\nlimitations: In-vitro cofactor titration; no dietary deficiency threshold was tested.\n[b6-shmt-2015] How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state (2015). https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.13211 DOI: 10.1111/febs.13211","model_system":"Purified human SHMT1 and SHMT2; structures and solution oligomerization","directness":"author_interpretation","verification_status":"source_derived_draft","notes":"Exact curation-document quotation, not publisher quotation. Study references: [b6-shmt-2015] How pyridoxal 5'-phosphate differentially regulates human cytosolic and mitochondrial serine hydroxymethyltransferase oligomeric state (2015). https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.13211 DOI: 10.1111/febs.13211","relationship":"supports","weight":1.0,"link_notes":"","source":{"id":"251773bb-16f5-5903-b135-db4a61d9dec4","stable_key":"import-1310afbd-6010-586e-805d-551d846da421","title":"Vitamin B6: mechanisms, deficiency and nutrient interactions (2026-09-17)","document_type":"imported_text","citation_label":"AI-assisted literature curation; primary study URLs and scope retained in the document and extraction. Not publisher full text.","file_path":"","sha256":"ef0019b344b2219220f801a84d0d138ff6880c1be1a59540d9034bfa4334f61e","revision_id":"cac3555f-48af-5c52-a84e-add4482c87fb","review_status":"unverified_draft","notes":""}}],"relations":[],"conflicts":[],"corrections":[],"research":null}